Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIFFERENTIAL LOCALIZATION OF CYTOPLASMIC MYOSIN-II ISOFORMS-A AND ISOFORMS-B IN AVIAN INTERPHASE AND DIVIDING EMBRYONIC AND IMMORTALIZED CARDIOMYOCYTES AND OTHER CELL-TYPES IN-VITRO
Autore:
CONRAD AH; JAFFREDO T; CONRAD GW;
Indirizzi:
KANSAS STATE UNIV AGR & APPL SCI,DIV BIOL,ACKERT HALL MANHATTAN KS 66506 CNRS,INST EMBRYOL CELLULAIRE & MOLEC F-94736 NOGENT SUR MARNE FRANCE COLL FRANCE NOGENT SUR MARNE FRANCE
Titolo Testata:
Cell motility and the cytoskeleton
fascicolo: 2, volume: 31, anno: 1995,
pagine: 93 - 112
SICI:
0886-1544(1995)31:2<93:DLOCMI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED CARDIAC MYOCYTES; HEAVY-CHAIN; NONMUSCLE MYOSIN; ACTOMYOSIN ORGANIZATION; MAMMALIAN-CELLS; MESSENGER-RNAS; ALPHA-ACTININ; EXPRESSION; CYTOKINESIS; IDENTIFICATION;
Keywords:
CLEAVAGE FURROW; CYTOKINESIS; CONTRACTILE RING; MICROFILAMENT STRESS FIBERS; MICROFILAMENT NETWORKS; INTESTINAL EPITHELIUM; SPLEEN CELLS; DORSAL ROOT GANGLIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
A.H. Conrad et al., "DIFFERENTIAL LOCALIZATION OF CYTOPLASMIC MYOSIN-II ISOFORMS-A AND ISOFORMS-B IN AVIAN INTERPHASE AND DIVIDING EMBRYONIC AND IMMORTALIZED CARDIOMYOCYTES AND OTHER CELL-TYPES IN-VITRO", Cell motility and the cytoskeleton, 31(2), 1995, pp. 93-112

Abstract

Two principal isoforms of cytoplasmic myosin II, A and B (CMIIA and CMIIB), are present in different proportions in different tissues. Isoform-specific monoclonal and polyclonal antibodies to avian CMIIA and CMIIB reveal the cellular distributions of these isoforms in interphaseand dividing embryonic avian cardiac, intestinal epithelial, spleen, and dorsal root ganglia cells in primary cell culture. Embryonic cardiomyocytes react with antibodies to CMIIB but not to CMIIA, localize CMIIB in stress-fiber-like-structures during interphase, and markedly concentrate CMIIB in networks in the cleavage furrow during cytokinesis. In contrast, cardiac fibroblasts localize both CMIIA and CMIIB in stress fibers and networks during interphase, and demonstrate slight and independently regulated concentration of CMIIA and CMIIB in networks in their cleavage furrows. V-myc-immortalized cardiomyocytes, an established cell line, have regained the ability to express CMIIA, as well as CMIIB, and localize both CMIIA and CMIIB in stress fibers and networks in interphase cells and in cleavage furrows in dividing cells. Conversely, some intestinal epithelial, spleen, and dorsal root ganglia interphase cells express only CMIIA, organized primarily in networks. Ofthese, intestinal epithelial cells express both CMIIA and CMIIB when they divide, whereas some dividing cells from both spleen and dorsal root ganglia express only CMIIA and concentrate it in their cleavage furrows. These results suggest that within a given tissue, different cell types express different isoforms of CMII, and that cells expressing either CMIIA or CMIIB alone, or simultaneously, can form a cleavage furrow and divide. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:45:22