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Titolo:
SELECTIVE VARIATIONS IN-VIVO OF V(H)3 AND V(H)1 GENE FAMILY EXPRESSION IN PERIPHERAL B-CELL IGM, IGD AND IGG DURING HIV-INFECTION
Autore:
DAVID D; DEMAISON C; BANI L; ZOUALI M; THEZE J;
Indirizzi:
INST PASTEUR,DEPT IMMUNOL,UNITE IMMUNOGENET CELLULAIRE,25 RUE DR ROUXF-75724 PARIS 15 FRANCE INST PASTEUR,DEPT IMMUNOL,UNITE IMMUNOGENET CELLULAIRE F-75724 PARIS 15 FRANCE
Titolo Testata:
European Journal of Immunology
fascicolo: 6, volume: 25, anno: 1995,
pagine: 1524 - 1528
SICI:
0014-2980(1995)25:6<1524:SVIOVA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSITU HYBRIDIZATION; IMMUNOGLOBULIN-VH; LYMPHOCYTES; ANTIBODIES; REGION; INDIVIDUALS; STIMULATION; REPERTOIRE; SEGMENTS; LOCUS;
Keywords:
B CELL REPERTOIRE; HIV INFECTION; V-H GENE FAMILY; B CELL SUPERANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
D. David et al., "SELECTIVE VARIATIONS IN-VIVO OF V(H)3 AND V(H)1 GENE FAMILY EXPRESSION IN PERIPHERAL B-CELL IGM, IGD AND IGG DURING HIV-INFECTION", European Journal of Immunology, 25(6), 1995, pp. 1524-1528

Abstract

We have analyzed the expression of V-H gene families in IgM, IgD and IgG of peripheral blood B cells from a group of HIV-infected patients. CD19(+)CD20(+) cells were purified and anchored reverse transcriptase-polymerase chain reaction products were hybridized with V-H gene family probes. IgM, IgD and IgG that expressed a V(H)3 gene family segment, were decreased in patients with low CD4 counts and to a greater extend in patients with AIDS symptoms (up to 85 % for IgG) compared to adult healthy donors. This was correlated with elevated levels of IgM andIgG encoded by a V(H)1 gene family segment (around 60 % for IgG). These results confirm and extend previous work that has detected the V(H)3 gene family under-representation in HIV infection. Here, we show that. in vivo, this phenomenon actually affects the different B cell populations of the peripheral blood: IgM(+) or IgG(+) B cells and also IgM(+)IgD(+) naive B cells. In the course of HIV infection, this results in their gradual depletion. Data presented here strengthen the hypothesis that a B cell superantigen exists in HIV infection. These pronounced variations of the normally most-expressed V, gene family may be related to B cell abnormalities detected in HIV-infected patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:44:16