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Titolo:
SYNERGISTIC EFFECTS OF STEM-CELL FACTOR AND INTERLEUKIN-6 OR INTERLEUKIN-11 ON THE EXPANSION OF MURINE HEMATOPOIETIC PROGENITORS IN LIQUID SUSPENSION-CULTURE
Autore:
ARIYAMA Y; MISAWA S; SONODA Y;
Indirizzi:
KYOTO PREFECTURAL UNIV MED,DEPT HYG,KAMIGYO KU KYOTO 602 JAPAN KYOTO PREFECTURAL UNIV MED,DEPT HYG,KAMIGYO KU KYOTO 602 JAPAN KYOTO PREFECTURAL UNIV MED,DEPT INTERNAL MED 3 KYOTO 602 JAPAN
Titolo Testata:
Stem cells
fascicolo: 4, volume: 13, anno: 1995,
pagine: 404 - 413
SICI:
1066-5099(1995)13:4<404:SEOSFA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-KIT LIGAND; SERUM-FREE CULTURE; INTERFERON-GAMMA; FACTOR-I; GM-CSF; PROLIFERATION; GROWTH; IDENTIFICATION; EXPRESSION; DIFFERENTIATION;
Keywords:
SCF; IL-6; IL-11; IL-1-ALPHA; IFN-GAMMA; STEM CELL EXPANSION; CFU-MIX; HPP-CFC; BMT; PBSCT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
Y. Ariyama et al., "SYNERGISTIC EFFECTS OF STEM-CELL FACTOR AND INTERLEUKIN-6 OR INTERLEUKIN-11 ON THE EXPANSION OF MURINE HEMATOPOIETIC PROGENITORS IN LIQUID SUSPENSION-CULTURE", Stem cells, 13(4), 1995, pp. 404-413

Abstract

The synergistic effects of stem cell factor (SCF) in combination withother growth factors including interleukin (IL)-6, IL-11, IL-3, GM-CSF, G-CSF, IL-1 alpha and interferon-gamma (IFN-gamma) on the expansionof murine hematopoietic progenitors were studied in a short-term liquid suspension culture system. Bone marrow (BM) cells obtained 2 days after 5-fluorouracil (5-FU) injection were cultured for up to 18 days in serum-containing and serum-free cultures in the presence of combinations of various cytokines. The numbers of nucleated cells, total colony-forming cells (CFC), mixed-colony forming units (CPU-Mix) and high-proliferative potential colony-forming cells (HPP-CPC) before and afterliquid suspension cultures were measured in the presence of differentcombinations of cytokines. Combinations of SCF with IL-11, IL-6 or IL-1 alpha markedly increased the numbers of total CFC, CPU-Mix and HPP-CFC. A combination of SCP and IL-3 also expanded the number of total CFC; however, the fold increase was smaller than those of SCF plus IL-11, IL-6 or IL-1 alpha. Three or four factor combinations including SCFwith IL-3, IL-6 and IL-11 did not yield increased numbers of total CPC over that supported by SCP plus either IL-6 or IL-11. The addition of IFN-gamma to the culture containing SCP plus IL-11 resulted in a decrease of the expansion efficiency. However, this difference is not statistically significant. In contrast, the addition of IFN-gamma to the cultures containing SCF plus IL-6 did not affect the expansion efficiency. Interestingly, the addition of IL-1 alpha in the culture containing SCF plus IL-3 significantly increased the number of HPP-CFC over that supported by SCP plus IL-3 (p < 0.01). In contrast, IL-1 alpha did not significantly affect the expansion efficiency in the presence of SCF plus IL-6 or IL-11. These results suggest that combinations of SCF plus either IL-6 or IL-11 or a combination of SCF, IL-3 and IL-1 alphacan most effectively expand murine hematopoietic progenitors derived from day-2 post-5-FU BM cells in vitro.

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Documento generato il 21/09/20 alle ore 12:28:41