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Titolo:
THE EFFECT OF LIPID-PEROXIDATION AND LIPOLYSIS ON THE ABILITY OF LIPOPROTEINS TO INFLUENCE THROMBOPLASTIN ACTIVITY
Autore:
ETTELAIE C; HOWELL RM; BRUCKDORFER KR;
Indirizzi:
ROYAL FREE HOSP,SCH MED,DEPT BIOCHEM & MOLEC BIOL,ROWLAND HILL ST LONDON NW3 2PF ENGLAND UNIV LONDON KINGS COLL,DIV LIFE SCI,BIOCHEM SECT LONDON W8 7AH ENGLAND
Titolo Testata:
Biochimica et biophysica acta, L. Lipids and lipid metabolism
fascicolo: 1, volume: 1257, anno: 1995,
pagine: 25 - 30
SICI:
0005-2760(1995)1257:1<25:TEOLAL>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; CORONARY HEART-DISEASE; VII COAGULANT ACTIVITY; HUMAN MONOCYTE-MACROPHAGES; APOLIPOPROTEIN-B; TISSUE FACTOR; SCAVENGER RECEPTOR; OXIDATION; CELLS; INHIBITION;
Keywords:
LIPOPROTEIN; MODIFICATION; APOLIPOPROTEIN B; THROMBOPLASTIN; INHIBITION; THROMBOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
C. Ettelaie et al., "THE EFFECT OF LIPID-PEROXIDATION AND LIPOLYSIS ON THE ABILITY OF LIPOPROTEINS TO INFLUENCE THROMBOPLASTIN ACTIVITY", Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1257(1), 1995, pp. 25-30

Abstract

High, low and very low density lipoproteins and lipoprotein (a) were prepared from porcine serum. The apolipoprotein components of the lipoproteins were then isolated and resuspended in soybean lecithin. Apolipoprotein B was also resuspended in lipids more representative of those found in LDL and VLDL. Lipid peroxidation was induced in samples of all the lipoproteins and reconstituted apolipoproteins by incubation with either Cu2+ ions or hedgehog 15-lipoxygenase. Furthermore, aliquots of the samples were incubated with a mixture of lipases. The effect of native preparations and the treated samples on the procoagulant activity of thromboplastin was examined. Native HDL, apo A-II, native LDL, reconstituted LDL and apo B inhibited thromboplastin activity, whereas native VLDL and reconstituted VLDL enhanced this activity. While the ability of HDL and apolipoprotein A-II to inhibit thromboplastin wasunaltered by either Cu2+ oxidation, lipoxygenase oxidation or lipolysis, VLDL and particles resembling VLDL, which acted cooperatively withthromboplastin lost their activating potential. On the other hand, LDL and particles resembling LDL changed from being inhibitory to enhancing the thromboplastin activity following oxidation, but not after lipolysis. Apolipoprotein B fragments obtained by mild digestion of this protein, expressed an inhibitory effect towards thromboplastin, while extensive degradation of the protein reduced its inhibitory potential. It is suggested that modifications of lipoproteins in vivo can lead to a hypercoagulable state by modulation of the cofactor activity of thromboplastin to factor VII.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/02/20 alle ore 02:56:14