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Titolo:
DEVELOPMENTAL-CHANGES PREDISPOSE THE FETAL THYMUS TO POSITIVE SELECTION OF CD4(-) T-CELLS()CD8()
Autore:
FAIRCHILD PJ; AUSTYN JM;
Indirizzi:
UNIV CAMBRIDGE,DEPT PATHOL,TENNIS COURT RD CAMBRIDGE CB2 1QP ENGLAND UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT SURG OXFORD ENGLAND
Titolo Testata:
Immunology
fascicolo: 2, volume: 85, anno: 1995,
pagine: 292 - 298
SICI:
0019-2805(1995)85:2<292:DPTFTT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELLS; MOUSE THYMUS; THYMOCYTES; ONTOGENY; DIFFERENTIATION; POPULATIONS; ANTIBODIES; RUDIMENTS; IMMATURE; CULTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Fairchild e J.M. Austyn, "DEVELOPMENTAL-CHANGES PREDISPOSE THE FETAL THYMUS TO POSITIVE SELECTION OF CD4(-) T-CELLS()CD8()", Immunology, 85(2), 1995, pp. 292-298

Abstract

Selection of a competent T-cell repertoire is dependent on complex interactions between immature thymocytes and components of the thymic stroma. These events may be preserved in vitro by excising developing thymus rudiments and maintaining them under carefully controlled conditions in fetal thymus organ cultures (FTOC). Using this approach, we have shown that the ability of C57B1/6 thymi to sustain positive selection of mature CD4(+) CD8(-) cells is profoundly influenced by the day ofgestation on which they are excised: while thymocytes from day 14 rudiments fail to progress beyond the CD4(+) CD8(+) stage of the developmental pathway, day 15 and day 16 thymi support the differentiation of CD4(+) CD8(-) thymocytes. Importantly, day 16 thymocytes transferred to day 14 deoxyguanosine-treated rudiments are likewise arrested at theCD4(+) CD8(+) stage, suggesting that the thymic microenvironment of day 14 rudiments, rather than the state of differentiation of the thymocytes they contain, is responsible for the block in positive selection. Our studies of the stromal elements of day 14 rudiments have, however, revealed no obvious deficiencies in the cell types represented, or their expression of class II major histocompatibility complex (MHC) determinants. Furthermore, we have been unable to circumvent the blockage in positive selection by the addition of certain cytokines expressedlate during gestation. These results suggest that subtle changes occurring at day 15 of ontogeny render the thymic microenvironment capableof positive selection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 08:58:59