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Titolo:
EVIDENCE OF POSTTRANSCRIPTIONAL REGULATION OF L-SELECTIN GENE-EXPRESSION IN RAT LYMPHOID-CELLS
Autore:
SACKSTEIN R; MENG L; XU XM; CHIN YH;
Indirizzi:
UNIV S FLORIDA,COLL MED,H LEE MOFFITT CANC CTR,DEPT INTERNAL MED,DIV BONE MARROW TRANSPLANTAT TAMPA FL 33612 UNIV S FLORIDA,COLL MED,H LEE MOFFITT CANC CTR,DEPT PATHOL & LAB MED TAMPA FL 33612 UNIV MIAMI,SCH MED,DEPT MICROBIOL & IMMUNOL MIAMI FL 00000 VET AFFAIRS MED CTR MIAMI FL 33125
Titolo Testata:
Immunology
fascicolo: 2, volume: 85, anno: 1995,
pagine: 198 - 204
SICI:
0019-2805(1995)85:2<198:EOPROL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL VENULE CELLS; NODE HIGH ENDOTHELIUM; HOMING RECEPTOR; FACTOR-B; LYMPHOCYTES; ADHESION; SURFACE; RECOGNITION; GP90MEL-14; MOLECULES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
R. Sackstein et al., "EVIDENCE OF POSTTRANSCRIPTIONAL REGULATION OF L-SELECTIN GENE-EXPRESSION IN RAT LYMPHOID-CELLS", Immunology, 85(2), 1995, pp. 198-204

Abstract

Early investigations of lymphocyte migration in the rat operationallyidentified a lymphocyte membrane protein, designated 'A.11', which mediates lymphocyte adherence to lymph node (LN) high endothelial venules (HEV). To determine the primary structure of A.11 and examine its expression in lymphoid cells, we constructed an expression phage cDNA library of rat thoracic duct lymphocytes (TDL) and performed screening by immunoselection (utilizing an anti-A.11 polyclonal antiserum) as well as by hybridization selection. We have isolated a similar to 1.6 kb clone, RS-2, and sequencing revealed that it encodes rat L-selectin. The clone contains the complete coding sequence, a 105-bp 5' untranslated region and a 359-bp 3' untranslated region. Transfection of RS-2 cDNA into 70Z/3 cells conferred binding to HEV concomitant with expression of A.11, providing direct evidence that A.11 is rat L-selectin. Metabolic radiolabelling studies revealed that thymocytes synthesize markedly less L-selectin than do TDL or LN lymphocytes. However, Northern blot studies using RS-2 as a probe indicate that thymocytes possess more L-selectin RNA than does TDL. Together, these data provide evidencethat post-transcriptional events contribute to regulation of L-selectin expression in thymocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:57:11