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Titolo:
A GENETIC SCREEN IDENTIFIES CELLULAR FACTORS INVOLVED IN RETROVIRAL -1-FRAMESHIFTING
Autore:
LEE SI; UMEN JG; VARMUS HE;
Indirizzi:
NIH,BLDG 1,ROOM 126,9000 ROCKVILLE PIKE BETHESDA MD 20892 UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS SAN FRANCISCO CA 94143
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 14, volume: 92, anno: 1995,
pagine: 6587 - 6591
SICI:
0027-8424(1995)92:14<6587:AGSICF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; RIBOSOMAL-PROTEIN; RNA PSEUDOKNOT; FUSION PROTEIN; MESSENGER-RNA; RAM PROTEIN; YEAST; EXPRESSION; SUPPRESSOR; VIRUS;
Keywords:
TRANSLATION; CUPI; IFSI; PAROMOMYCIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S.I. Lee et al., "A GENETIC SCREEN IDENTIFIES CELLULAR FACTORS INVOLVED IN RETROVIRAL -1-FRAMESHIFTING", Proceedings of the National Academy of Sciences of the United Statesof America, 92(14), 1995, pp. 6587-6591

Abstract

To identify cellular factors that function in -1 ribosomal frameshifting, we have developed assays in the yeast Saccharomyces cerevisiae toscreen for host mutants in which frameshifting is specifically affected. Expression vectors have been constructed in which the mouse mammary tumor virus gag-pro frameshift region is placed upstream of the lacZgene or the CUP1 gene so that the reporters are in the -1 frame relative to the initiation codon, These vectors have been used to demonstrate that -1 frameshifting is recapitulated in yeast in response to retroviral mRNA signals, Using these reporters, we have isolated spontaneous host mutants in two complementation groups, ifs1 and ifs2, in whichframeshifting is increased 2-fold. These mutants are also hypersensitive to antibiotics that target the 40S ribosomal subunit, We have cloned the IFS1 gene and shown that it encodes a previously undescribed protein of 1091 aa with clusters of acidic residues in the carboxyl-terminal region, Haploid cells lacking 82% of the IFS1 open reading frame are viable and phenotypically identical to IFS1-1 mutants, This approach could help identify potential targets for antiretroviral agents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:03:26