Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MONOCLONAL-ANTIBODY HFD9 IDENTIFIES A NOVEL 28 KDA INTEGRAL MEMBRANE-PROTEIN ON THE CIS-GOLGI
Autore:
SUBRAMANIAM VN; KRIJNSELOCKER J; TANG BL; ERICSSON M; YUSOFF ARB; GRIFFITHS G; HONG WJ;
Indirizzi:
NATL UNIV SINGAPORE,INST MOLEC & CELL BIOL,MEMBRANE BIOL LAB,10 KENT RIDGE CRESCENT SINGAPORE 0511 SINGAPORE NATL UNIV SINGAPORE,INST MOLEC & CELL BIOL,MEMBRANE BIOL LAB SINGAPORE 0511 SINGAPORE EUROPEAN MOLEC BIOL LAB W-6900 HEIDELBERG GERMANY
Titolo Testata:
Journal of Cell Science
, volume: 108, anno: 1995,
parte:, 6
pagine: 2405 - 2414
SICI:
0021-9533(1995)108:<2405:MHIAN2>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE; ENDOPLASMIC-RETICULUM; BREFELDIN-A; LINKED OLIGOSACCHARIDES; APPARATUS; COMPLEX; NETWORK; EXPRESSION; TRANSPORT; PATHWAY;
Keywords:
GOLGI PROTEIN; MEMBRANE; P-28;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
V.N. Subramaniam et al., "MONOCLONAL-ANTIBODY HFD9 IDENTIFIES A NOVEL 28 KDA INTEGRAL MEMBRANE-PROTEIN ON THE CIS-GOLGI", Journal of Cell Science, 108, 1995, pp. 2405-2414

Abstract

We have raised a monoclonal antibody (mAb) (HFD9) that detects a 28 kDa protein (p28) enriched in the Golgi membrane. p28 was localized to the perinuclear Golgi region in all cell lines thus far examined. Its Golgi localization was confirmed by its colocalization with Golgi markers using indirect immunofluorescence microscopy. Immunogold labellingdemonstrates that the majority of p28 was localized on the cis-Golgi and its associated structures. Two independent experiments demonstratethat the p28 epitope recognized by mAb HFD9 is exposed to the cytosol. Extraction of Golgi membranes with a variety of reagents revealed that p28 behaves like an integral membrane protein. mAb HFD9 thus defines a novel 28 kDa integral membrane protein on the cis-Golgi. To our knowledge, p28 represents the first integral membrane protein of the Golgi system identified via the antibody approach whose epitope is cytoplasmically-oriented and highly-conserved. Monoclonal antibody HFD9 willthus provide a useful tool for further studies on the cis side of theGolgi, which is not well characterised due to the lack of good markers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 00:45:36