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Titolo:
CHARACTERIZATION OF A DESMOCOLLIN ISOFORM (BOVINE DSC3) EXCLUSIVELY EXPRESSED IN LOWER LAYERS OF STRATIFIED EPITHELIA
Autore:
YUE KKM; HOLTON JL; CLARKE JP; HYAM JLM; HASHIMOTO T; CHIDGEY MAJ; GARROD DR;
Indirizzi:
UNIV MANCHESTER,SCH BIOL SCI,EPITHELIAL MORPHOGENESIS RES GRP,CANC RES CAMPAIGN MANCHESTER M13 9PT LANCS ENGLAND UNIV MANCHESTER,SCH BIOL SCI,EPITHELIAL MORPHOGENESIS RES GRP,CANC RES CAMPAIGN MANCHESTER M13 9PT LANCS ENGLAND LEICESTER ROYAL INFIRM,DEPT HISTOPATHOL LEICESTER LE1 5WW LEICS ENGLAND KEIO UNIV,SCH MED,DEPT DERMATOL TOKYO JAPAN
Titolo Testata:
Journal of Cell Science
, volume: 108, anno: 1995,
parte:, 6
pagine: 2163 - 2173
SICI:
0021-9533(1995)108:<2163:COADI(>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION MOLECULES; DESMOSOMAL CADHERINS; GLYCOPROTEIN-3 DESMOCOLLINS; CYTOPLASMIC DOMAINS; HUMAN EPIDERMIS; SEQUENCE; ACID; IDENTIFICATION;
Keywords:
DESMOSOME; DESMOCOLLIN; RT-PCR; EXPRESSION; STRATIFIED EPITHELIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
K.K.M. Yue et al., "CHARACTERIZATION OF A DESMOCOLLIN ISOFORM (BOVINE DSC3) EXCLUSIVELY EXPRESSED IN LOWER LAYERS OF STRATIFIED EPITHELIA", Journal of Cell Science, 108, 1995, pp. 2163-2173

Abstract

Desmocollins are cadherin-like glycoproteins involved in cell adhesion and plaque formation in desmosome junctions. Three distinct isoforms, the products of different genes, have been found in bovine tissues. We have reported previously that one of these, DSC3, is expressed onlyin basal and lower suprabasal layers of stratified epithelia. Using RT-PCR we have now obtained the complete cDNA coding sequence of maturebovine DSC3. It has alternatively spliced 'a' and 'b' forms found in other desmocollins but is unique in having a 43 instead of a 46 base pair exon. We have characterised a monoclonal antibody, 07-4G, which isspecific for the Dsc3 protein, recognising an epitope in the extracellular domain. Immunofluorescent staining with 07-4G confirms that thisisoform is found only in stratified epithelia, being strongly expressed in the basal cell layers of these tissues. The intensity of expression fades gradually in the suprabasal layers and disappears completelybelow the upper limit of desmosome expression, These results suggest that Dsc3 plays an important role in cell epithelial differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:04:06