Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SELECTIVE OXIDATION - STABILIZATION BY MULTIPOINT ATTACHMENT OF FERREDOXIN NADP(+) REDUCTASE, AN INTERESTING COFACTOR RECYCLING ENZYME
Autore:
BES MT; GOMEZMORENO C; GUISAN JM; FERNANDEZLAFUENTE R;
Indirizzi:
UNIV AUTONOMA MADRID,CSIC,INST CATALISIS,TECNOL ENZIMAT LAB,CANTO BLANCO E-28049 MADRID SPAIN UNIV AUTONOMA MADRID,CSIC,INST CATALISIS,TECNOL ENZIMAT LAB E-28049 MADRID SPAIN UNIV ZARAGOZA,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC & CELULAR E-50009 ZARAGOZA SPAIN
Titolo Testata:
Journal of molecular catalysis. A, Chemical
fascicolo: 3, volume: 98, anno: 1995,
pagine: 161 - 169
SICI:
1381-1169(1995)98:3<161:SO-SBM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PENICILLIN-G ACYLASE; ALDEHYDE-AGAROSE GELS; IMMOBILIZATION-STABILIZATION; ORGANIC-SYNTHESIS; NICOTINAMIDE COFACTORS; COVALENT ATTACHMENT; REGENERATION; PURIFICATION;
Keywords:
COFACTOR REGENERATION; ENZYME STABILIZATION; ENZYMATIC OXIDATION; FERREDOXIN-NADP(+) REDUCTASE; IMMOBILIZATION OF ENZYMES; REDUCTASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
M.T. Bes et al., "SELECTIVE OXIDATION - STABILIZATION BY MULTIPOINT ATTACHMENT OF FERREDOXIN NADP(+) REDUCTASE, AN INTERESTING COFACTOR RECYCLING ENZYME", Journal of molecular catalysis. A, Chemical, 98(3), 1995, pp. 161-169

Abstract

Ferredoxin-NADP(+) reductase (FNR, EC 1.18.1.2) is an enzyme that is able to catalyse the oxidation of NADPH + H+. A strategy to prepare industrial derivatives of this enzyme for use as an 'NADP' regenerating enzyme in oxidizing reactions is presented. The strategy is based on astrictly controlled process of multipoint covalent attachment betweenthe enzyme, via its amino groups, and a pre-existing solid activated with a monolayer of simple aldehyde groups linked by a space-arm of moderate length to the surface of the support. Controlling the variableswhich may have an influence in the multi-interaction process, we haveprepared a number of enzyme derivatives with very different activity/stability properties. The 'optimum derivative' was found to be much more stable than its corresponding soluble enzyme under all the denaturation conditions assayed (high temperatures, extreme pH, organic solvents, etc.). Because of the excellent properties of this enzyme derivative, we can regenerate NADP(+) by using molecular oxygen directly as the oxidizing agent under a wide range of conditions. Coupling this oxidative system to other NADP-dependent redox enzymes, we should be able to develop a very specific and selective oxidative procedure under very mild oxidizing conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 05:19:53