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Titolo:
AMPHOTROPIC AND ECOTROPIC RETROVIRAL VECTOR VIRUSES TRANSDUCE MIDGESTATIONAL MURINE FETAL LIVER-CELLS IN A DUAL-CHAMBERED COCULTIVATION SYSTEM
Autore:
CASAL ML; WOLFE JH;
Indirizzi:
UNIV PENN,SCH VET MED,DEPT PATHOBIOL,3800 SPRUCE ST PHILADELPHIA PA 19104 UNIV PENN,SCH VET MED,DEPT PATHOBIOL PHILADELPHIA PA 19104
Titolo Testata:
Gene therapy
fascicolo: 1, volume: 4, anno: 1997,
pagine: 39 - 44
SICI:
0969-7128(1997)4:1<39:AAERVV>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUCOPOLYSACCHARIDOSIS TYPE-VII; HEMATOPOIETIC STEM-CELLS; BETA-GLUCURONIDASE DEFICIENCY; MEDIATED GENE-TRANSFER; BONE-MARROW TRANSPLANTATION; LYSOSOMAL STORAGE; EXPRESSION; MICE; MOUSE; RECEPTORS;
Keywords:
MURINE FETAL LIVER CELLS; AMPHOTROPIC RETROVIRUS; ECOTROPIC RETROVIRUS; DUAL-CHAMBERED COCULTIVATION; IN UTERO TRANSPLANTATION; MUCOPOLYSACCHARIDOSIS TYPE VII;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
M.L. Casal e J.H. Wolfe, "AMPHOTROPIC AND ECOTROPIC RETROVIRAL VECTOR VIRUSES TRANSDUCE MIDGESTATIONAL MURINE FETAL LIVER-CELLS IN A DUAL-CHAMBERED COCULTIVATION SYSTEM", Gene therapy, 4(1), 1997, pp. 39-44

Abstract

A beta-glucuronidase cDNA was transferred into fetal liver cells (FLC) from mice affected with mucopolysaccharidosis (MPS) type VII and normal littermates using a retrovirus vector. The cells were transduced by direct cocultivation or by culturing the FLC and vector packaging cells separated by a 0.45 mu m filter in a dual-chambered cocultivation system. Gene transduction occurred using an ecotropic or amphotropic vector in FLC obtained from 13.5- and 15.5-murine day-old murine fetuses. Histochemical staining assays and measurement of enzyme activity demonstrated gene expression in the midgestational FLC. Enzyme secreted into the supernatant from transduced FLC obtained from 13.5-day-old affected fetuses surpassed secreted enzyme from normal, age-matched untransduced FLC. The 13.5 day FLC, transduced by direct cocultivation or by using the dual-chambered system, were transplanted in utero into 13.5-day-old murine fetuses. Proviral sequences were detected in variousorgans shortly after birth. The results indicate that midgestational FLC can be transduced with an amphotropic vector virus in a dual-chambered cocultivation system without contaminating virus-producing packaging cells and that the transduced cells survive in utero transplantation.

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Documento generato il 25/11/20 alle ore 08:56:17