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Titolo:
ANAEROBIC OXIDATION OF TOLUENE (ANALOGS) TO BENZOATE (ANALOGS) BY WHOLE CELLS AND BY CELL-EXTRACTS OF A DENITRIFYING THAUERA SP
Autore:
BIEGERT T; FUCHS G;
Indirizzi:
UNIV ULM D-89069 ULM GERMANY UNIV ULM D-89069 ULM GERMANY UNIV FREIBURG,INST BIOL 2,LEHRSTUHL MIKROBIOL D-79104 FREIBURG GERMANY
Titolo Testata:
Archives of microbiology
fascicolo: 6, volume: 163, anno: 1995,
pagine: 407 - 417
SICI:
0302-8933(1995)163:6<407:AOOT(T>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
BENZYL ALCOHOL-DEHYDROGENASE; SULFATE-REDUCING CONDITIONS; BENZALDEHYDE DEHYDROGENASE; ACINETOBACTER-CALCOACETICUS; MICROBIAL-DEGRADATION; ENZYMATIC REDUCTION; AROMATIC METABOLISM; PSEUDOMONAS-PUTIDA; PHENOLIC-COMPOUNDS; MOLECULAR-OXYGEN;
Keywords:
THAUERA; TOLUENE; BENZYL ALCOHOL; TOLUENE-OXIDIZING ENZYME SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
T. Biegert e G. Fuchs, "ANAEROBIC OXIDATION OF TOLUENE (ANALOGS) TO BENZOATE (ANALOGS) BY WHOLE CELLS AND BY CELL-EXTRACTS OF A DENITRIFYING THAUERA SP", Archives of microbiology, 163(6), 1995, pp. 407-417

Abstract

Toluene and related aromatic compounds can be mineralized to CO, under anoxic conditions. Oxidation requires new dehydrogenase-type enzymesand water as oxygen source, as opposed to the aerobic enzymatic attack by oxygenases, which depends on molecular oxygen. We studied the anaerobic process in the denitrifying bacterium Thauera sp. strain K172. Toluene and a number of its fluoro-, chloro- and methyl-analogues weretransformed to benzoate and the respective analogues by whole cells and by cell extracts. The transformation of xylene isomers to methylbenzoate isomers suggests that xylene degradation is similarly initiated by oxidation of one of the methyl groups. Toluene oxidation was strongly, but reversibly inhibited by benzyl alcohol. The in vitro oxidationof the methyl group was coupled to the reduction of nitrate, requiredglycerol for activity, and was inhibited by oxygen. Cells also contained benzyl alcohol dehydrogenase (NAD(+)), benzaldehyde dehydrogenase (NADP(+)), benzoate-CoA ligase (AMP-forming), and benzoyl-CoA reductase (dearomatizing). The toluene-oxidizing activity was induced when cells were grown anaerobically with toluene and also with benzyl alcohol or benzaldehyde, suggesting that benzyl alcohol or benzaldehyde acts as inducer. The other enzymes were similarly active in cells grown withtoluene, benzyl alcohol, benzaldehyde, or benzoate. This is the firstin vitro study of anaerobic oxidation of an aromatic hydrocarbon and of the whole-cell regulation of the toluene-oxidizing enzyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 19:12:00