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Titolo:
CHARACTERIZATION OF A TN4351-GENERATED HEMIN UPTAKE MUTANT OF PORPHYROMONAS-GINGIVALIS - EVIDENCE FOR THE COORDINATE REGULATION OF VIRULENCE FACTORS BY HEMIN
Autore:
GENCO CA; SIMPSON W; FORNG RY; EGAL M; ODUSANYA BM;
Indirizzi:
MOREHOUSE SCH MED,DEPT MICROBIOL & IMMUNOL,720 WESTVIEW DR SW ATLANTAGA 30310
Titolo Testata:
Infection and immunity
fascicolo: 7, volume: 63, anno: 1995,
pagine: 2459 - 2466
SICI:
0019-9567(1995)63:7<2459:COATHU>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; PIGMENTED BACTEROIDES; HEMOLYTIC-ACTIVITY; ESCHERICHIA-COLI; EXTRACELLULAR VESICLES; MEMBRANE-VESICLES; SURFACE PROTEIN; ENZYME-ACTIVITY; W50; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Genco et al., "CHARACTERIZATION OF A TN4351-GENERATED HEMIN UPTAKE MUTANT OF PORPHYROMONAS-GINGIVALIS - EVIDENCE FOR THE COORDINATE REGULATION OF VIRULENCE FACTORS BY HEMIN", Infection and immunity, 63(7), 1995, pp. 2459-2466

Abstract

The ability of Porphyromonas gingivalis to acquire iron in the iron-limited environment of the host is crucial to the colonization of this organism. We report here on the isolation and characterization of a transpositional insertion mutant of P. gingivalis A7436 (designated MSM-3) which is defective in the utilization and transport of hemin. P. gingivalis MSM-3 was selected on the basis of its nonpigmented phenotypeon anaerobic blood agar following mutagenesis with the Bacteroides fragilis transposon Tn4351. P. gingivalis MSM-3 grew poorly when supplied with hemin as a sole source of iron; however, growth was observed with hemoglobin or inorganic iron. P. gingivalis MSM-3 grown in either hemin-replete or hemin-depleted conditions bound and transported less [C-14]hemin or [Fe-59]hemin than did the parent strain. At 4 h, P. gingivalis MSM-3 grown in hemin-replete conditions transported only 10,000pmol of hemin per mg of protein, or 14% of the amount transported by P. gingivalis A7436, Unlike P. gingivalis A7436, hemin binding and transport by P. gingivalis MSM-3 were not tightly regulated by hemin or iron. Examination of P. gingivalis MSM-3 cultures by electron microscopy revealed an overproduction of membrane vesicles, and determination of the dry weight of purified vesicles indicated that P. gingivalis MSM-3 produced twice as much membrane vesicles as did strain A7436. Extracellular vesicles isolated from P. gingivalis MSM-3 also were found toexpress increased hemolytic and trypsin-like protease activities compared with the parent strain. When inoculated into subcutaneous chambers implanted in mice, P. gingivalis MSM-3 was highly infectious and more invasive than the parent strain, as indicated by secondary lesion formation and death. Taken together, these results indicate that the decreased transport of hemin by P. gingivalis MSM-3 results in the increased expression of several virulence factors which may be coordinately regulated by hemin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:19:21