Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECTS OF THE SIZE AND FLUIDITY OF LIPOSOMES ON THEIR ACCUMULATION IN TUMORS - A PRESUMPTION OF THEIR INTERACTION WITH TUMORS
Autore:
UCHIYAMA K; NAGAYASU A; YAMAGIWA Y; NISHIDA T; HARASHIMA H; KIWADA H;
Indirizzi:
UNIV TOKUSHIMA,FAC PHARMACEUT SCI,DEPT PHARMACODYNAM & PHARMACEUT,SHOMACHI 1-78 TOKUSHIMA 770 JAPAN UNIV TOKUSHIMA,FAC PHARMACEUT SCI,DEPT PHARMACODYNAM & PHARMACEUT TOKUSHIMA 770 JAPAN TAISHO PHARMACEUT CO LTD,PHARMACEUT RES LAB TOKUSHIMA 77101 JAPAN
Titolo Testata:
International journal of pharmaceutics
fascicolo: 2, volume: 121, anno: 1995,
pagine: 195 - 203
SICI:
0378-5173(1995)121:2<195:EOTSAF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
STERICALLY STABILIZED LIPOSOMES; BLOOD; FLOW;
Keywords:
LIPOSOME; AUG; TUMOR UPTAKE CLEARANCE; ANGIOTENSIN II; YOSHIDA SARCOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
K. Uchiyama et al., "EFFECTS OF THE SIZE AND FLUIDITY OF LIPOSOMES ON THEIR ACCUMULATION IN TUMORS - A PRESUMPTION OF THEIR INTERACTION WITH TUMORS", International journal of pharmaceutics, 121(2), 1995, pp. 195-203

Abstract

The distribution of liposomes with different membrane fluidity and vesicle size in tumors after intravenous injection was investigated in Yoshida sarcoma-bearing rats. Liposomes composed of egg phosphatidylcholine (EPC) or hydrogenated egg phosphatidylcholine (HEPC), dicetyl phosphate and cholesterol in a molar ratio of 5:1:4 were prepared. Their size was adjusted so that they had various mean diameters, ranging from 40 to 400 nm. In EPC liposomes, whose membranes were more fluid thanthose of HEPC liposomes, tumor accumulation increased with increasingarea under the blood concentration-time curve (AUG). The size of liposomes which showed the greatest tumor accumulation and AUC was around 100 nm in diameter. In HEPC liposomes, the less fluid type, the size dependence of tumor accumulation and AUC differed. The greatest tumor accumulation or AUC were found in liposomes with a diameter of about 100 or 40 nm, respectively. This discrepancy indicates that the tumor accumulation of liposomes is not always correlated with their circulation time in the blood. To clarify the process by which these liposomes accumulate from the vascular space into the tumor, we calculated tumor uptake clearance (CL(tu)), which can separate the contribution of the blood concentration from the accumulation in tumor. The CL(tu) values for EPC and HEPC liposomes agreed at all sizes, liposomes with a diameter of 100 nm showing the highest values. These findings indicate thatthe accumulation of liposomes from the vascular space into the tumor is primarily governed by their size and not by their membrane fluidityor blood circulation time. When tumor blood flow was selectively enhanced by the infusion of angiotensin II, the CL(tu) of 100-nm liposomesdecreased to the level of that in 40-nm liposomes, suggesting that some histological factor(s) in the tumor may be responsible for the localization of 100-nm liposomes in tumor. In an in vitro experiment usingcultured Yoshida sarcoma cells, 59-nm HEPC liposomes were directly taken up by the tumor cells to an extent at least 2.5-times greater thanlarger liposomes (greater than or equal to 100 nm). We conclude that 100-nm liposomes may predominantly localize in the interstitial space,whereas some liposomes of smaller size may be taken up by tumor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/21 alle ore 15:16:38