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Titolo:
THE USE OF BRAIN-SLICES AND DISSOCIATED NEURONS TO EXPLORE THE MULTIPLICITY OF ACTIONS OF 5-HT IN THE CENTRAL-NERVOUS-SYSTEM
Autore:
LARKMAN PM; KELLY JS;
Indirizzi:
UNIV EDINBURGH,DEPT PHARMACOL,1 GEORGE SQ EDINBURGH EH8 9JZ MIDLOTHIAN SCOTLAND
Titolo Testata:
Journal of neuroscience methods
fascicolo: 1, volume: 59, anno: 1995,
pagine: 31 - 39
SICI:
0165-0270(1995)59:1<31:TUOBAD>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DORSAL RAPHE NEURONS; RAT SPINAL MOTONEURONS; ADULT-RAT; SEROTONERGIC NEURONS; POTASSIUM CHANNELS; INVITRO; 5-HYDROXYTRYPTAMINE; RECEPTORS; CURRENTS; NUCLEUS;
Keywords:
5-HT; DORSAL RAPHE NEURON; FACIAL MOTONEURON; POTASSIUM CHANNEL; I-H; BRAIN SLICE; DISSOCIATED NEURON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
P.M. Larkman e J.S. Kelly, "THE USE OF BRAIN-SLICES AND DISSOCIATED NEURONS TO EXPLORE THE MULTIPLICITY OF ACTIONS OF 5-HT IN THE CENTRAL-NERVOUS-SYSTEM", Journal of neuroscience methods, 59(1), 1995, pp. 31-39

Abstract

The cellular actions of 5-hydroxytryptamine (5-HT) on adult and neonatal rat central neurones have been investigated in detail using a combination of in vitro slice and dissociated neurone preparations. Patch-clamp recordings from acutely dissociated adult rat dorsal raphe neurones confirm data obtained using conventional slice preparations that 5-HT activates an inwardly rectifying potassium channel through a 5-HT1A receptor leading to hyperpolarization of the cell. Single-channel recordings indicate that this pathway requires only the involvement of apertussis toxin-sensitive G-protein. Adult rat facial motoneurones inconventional slices are depolarized by 5-HT through a combination of mechanisms, closure of potassium channels and enhancement of the hyperpolarization-activated, cationic current, I-H. Distinct receptors appear to mediate these two actions. Both mechanisms are present in visually indentified neonatal rat facial motoneurones in thin brain slices. Whole-cell patch-clamp recordings show the action of 5-HT on I-H to mediate a caesium-sensitive inward current which can be mimicked by the adenylate cyclase activator, forskolin. The experimental data illustrate how a range of complimentary in vitro electrophysiological techniques can be employed to unravel neurotransmitter mechanisms and pharmacology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:11:15