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Titolo:
CLONING OF A TRYPANOSOMATID GENE CODING FOR AN ORNITHINE DECARBOXYLASE THAT IS METABOLICALLY UNSTABLE EVEN THOUGH IT LACKS THE C-TERMINAL DEGRADATION DOMAIN
Autore:
SVENSSON F; CERIANI C; WALLSTROM EL; KOCKUM I; ALGRANATI ID; HEBY O; PERSSON L;
Indirizzi:
LUND UNIV,DEPT PHYSIOL & NEUROSCI,SOLVEGATAN 19 S-22362 LUND SWEDEN LUND UNIV,DEPT PHYSIOL & NEUROSCI S-22362 LUND SWEDEN UNIV BUENOS AIRES,INST INVEST BIOQUIM,FDN CAMPOMAR RA-1405 BUENOS AIRES DF ARGENTINA CONSEJO NACL INVEST CIENT & TECN RA-1405 BUENOS AIRES DF ARGENTINA UMEA UNIV,DEPT CELLULAR & DEV BIOL S-90187 UMEA SWEDEN
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 2, volume: 94, anno: 1997,
pagine: 397 - 402
SICI:
0027-8424(1997)94:2<397:COATGC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR DEGRADATION; CRITHIDIA-FASCICULATA; MOLECULAR-CLONING; MESSENGER-RNA; LEISHMANIA-DONOVANI; PEST HYPOTHESIS; INITIATION SITE; ANTIZYME; SEQUENCE; BRUCEI;
Keywords:
CRITHIDIA FASCICULATA; PROTEIN TURNOVER; POLYAMINES; PEST REGION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
F. Svensson et al., "CLONING OF A TRYPANOSOMATID GENE CODING FOR AN ORNITHINE DECARBOXYLASE THAT IS METABOLICALLY UNSTABLE EVEN THOUGH IT LACKS THE C-TERMINAL DEGRADATION DOMAIN", Proceedings of the National Academy of Sciences of the United Statesof America, 94(2), 1997, pp. 397-402

Abstract

Mammalian ornithine decarboxylase (ODC) is among the most labile of cellular proteins, with a half-life of usually less than an hour, Like other short-lived proteins ODC is degraded by the 26S proteasome, Its degradation is not triggered by ubiquitination, but is stimulated by the binding of an inducible protein, antizyme, Truncations and mutations in the C terminus of mammalian ODC have been shown to prevent the rapid turnover of the enzyme, demonstrating the presence of a degradation signal in this region, Moreover, ODCs from the trypanosomatid parasites Trypanosoma brucei and Leishmania donovani, which lack this C-terminal domain, are metabolically stable, and recombination of T. brucei ODC with the C terminus of mammalian ODC confers a short half-life to the fusion protein when expressed in mammalian cells, In the present study we have cloned and sequenced the ODC gene from the trypanosomatidCrithidia fasciculata, To our knowledge, this is the first protozoan shown to have an ODC with a rapid turnover, The sequence analysis revealed a high homology between C. fasciculata ODC and L. donovani ODC, despite the difference in stability, We demonstrate that C. fasciculataODC has a very rapid turnover even when expressed in mammalian tells,Moreover, ODC from C. fasciculata is shown to lack the C-terminal degradation domain of mammalian ODC, Our findings indicate that C. fasciculata ODC contains unique signals, targeting the enzyme for rapid degradation not only in the parasite but also in mammalian cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 01:00:04