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Titolo:
BASIC FIBROBLAST GROWTH-FACTOR - THE NEUROTROPHIC FACTOR INFLUENCING THE INGROWTH OF NEURAL TISSUE INTO THE ANTERIOR-PITUITARY OF ALPHA-T7 TRANSGENIC MICE
Autore:
SCHECHTER J; STAUBER C; WINDLE JJ; MELLON P;
Indirizzi:
UNIV SO CALIF,SCH MED,DEPT CELL & NEUROBIOL,1333 SAN PABLO ST LOS ANGELES CA 90033 UNIV CALIF SAN DIEGO,DEPT REPROD BIOL LA JOLLA CA 00000 UNIV CALIF SAN DIEGO,DEPT NEUROSCI LA JOLLA CA 00000
Titolo Testata:
Neuroendocrinology
fascicolo: 6, volume: 61, anno: 1995,
pagine: 622 - 627
SICI:
0028-3835(1995)61:6<622:BFG-TN>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; DNA-SYNTHESIS; T-ANTIGEN; TUMORS; MEMBRANES; CELLS;
Keywords:
TRANSGENIC MICE; TUMORS; GONADOTROPES; NEURONAL GROWTH; GROWTH FACTORS; ALPHA-T7 CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J. Schechter et al., "BASIC FIBROBLAST GROWTH-FACTOR - THE NEUROTROPHIC FACTOR INFLUENCING THE INGROWTH OF NEURAL TISSUE INTO THE ANTERIOR-PITUITARY OF ALPHA-T7 TRANSGENIC MICE", Neuroendocrinology, 61(6), 1995, pp. 622-627

Abstract

alpha-T7 mice are a transgenic line which carries a hybrid transgene composed of the 5' flanking region of the human glycoprotein hormone alpha-subunit gene (1.8 kb) linked to the coding region of the oncogeneSV40 T-antigen. Large, hemorrhagic, pituitary tumors form in these mice and contain giant, transformed gonadotropes (immunopositive for T-antigen), in addition to normal-appearing gonadotropes (also immunopositive for T-antigen). An additional feature of these tumors is an abundance of neural tissue proliferating throughout the anterior pituitary,concentrated around the giant gonadotropes, and forming synaptoid contacts upon them. Continued study of these mice has demonstrated that the giant gonadotropes contain immunostainable basic fibroblast growth factor (FGF-2), and apparently release the FGF by focal cellular disruption and/or cytoplasmic blebbing. Normal gonadotropes, in control andtransgenic mice, were strongly immunopositive for FGF, and appeared intact. In 8- to 13-month-old transgenic mice most of the giant cells were intact, and were surrounded by well-differentiated neural tissue. These giant cells were lightly immunopositive for FGF. Disrupted, giant gonadotropes were more frequent in 2- to 7-month-old transgenic mice, and also were surrounded by well-differentiated neural tissue with many synaptoid contacts. These cells generally were moderately immunopositive for FGF. In neonatal mice, 1-8 days old, precursors of the giant, transformed gonadotropes were identified, primarily, but not exclusively, near the periphery of the anterior pituitary. These cells stained strongly immunopositive for FGF, frequently were focally disrupted,and were associated with slender processes of neural tissue, but no synaptoid contacts. Mouse pituitaries, control and transgenic, were consistently immunonegative for nerve growth factor. We conclude that FGFis present within normal and transformed gonadotropes in alpha-T7 mice, and that FGF released from giant gonadotropes early in development appears likely to be the neurotrophic factor which induces neural tissue ingrowth into the anterior pituitaries of these mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:00:51