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Titolo:
FUNCTIONAL-ROLE AND SEQUENCE-ANALYSIS OF A LYMPHOCYTE ORPHAN OPIOID RECEPTOR
Autore:
HALFORD WP; GEBHARDT BM; CARR DJJ;
Indirizzi:
LOUISIANA STATE UNIV,MED CTR,DEPT MICROBIOL,1901 PERDIDO ST NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,DEPT MICROBIOL NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,DEPT IMMUNOL NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,DEPT PARASITOL NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,DEPT OPHTHALMOL NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,DEPT PHARMACOL NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,CTR NEUROSCI NEW ORLEANS LA 70112
Titolo Testata:
Journal of neuroimmunology
fascicolo: 1-2, volume: 59, anno: 1995,
pagine: 91 - 101
SICI:
0165-5728(1995)59:1-2<91:FASOAL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MORPHINE-INDUCED SUPPRESSION; IMMUNE-SYSTEM; ANTIBODY-RESPONSE; BETA-ENDORPHIN; OPIATE BINDING; DELTA-CLASS; CELLS; CLONING; EXPRESSION; ENHANCEMENT;
Keywords:
LYMPHOCYTE; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; ORPHAN OPIOID RECEPTOR; ANTISENSE OLIGONUCLEOTIDE; ALTERNATIVE SPLICING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
W.P. Halford et al., "FUNCTIONAL-ROLE AND SEQUENCE-ANALYSIS OF A LYMPHOCYTE ORPHAN OPIOID RECEPTOR", Journal of neuroimmunology, 59(1-2), 1995, pp. 91-101

Abstract

Pharmacological evidence indicates that lymphocytes express opioid receptors, but this finding has been questioned. By DNA sequencing of reverse transcription-polymerase chain reaction products, we have found that mouse lymphocytes express mRNA encoding an orphan opioid receptor. These mRNA transcripts were detected in the CD4(+), CD8(+), and CD4(-)CD8- lymphocyte subpopulations. Northern blot analysis confirmed that splenic lymphocytes express a 1.5-kb orphan opioid receptor mRNA. Fifteen bases encoding Tyr(71)-Arg(75) in the first intracellular loop are alternatively spliced, suggesting that orphan opioid receptor mRNA encodes two receptor subtypes. Treatment of lipopolysaccharide-stimulated lymphocytes with orphan opioid receptor antisense oligonucleotidessuppressed polyclonal IgG and IgM production by 50%. Our results provide direct evidence that lymphocytes express an opioid-like receptor gene, and suggest that this receptor plays a functional role in immunocompetence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:43:31