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Titolo:
PROFILE OF IMMUNOGLOBULIN HEAVY-CHAIN VARIABLE GENE REPERTOIRES AND HIGHLY SELECTIVE DETECTION OF MALIGNANT CLONOTYPES IN ACUTE LYMPHOBLASTIC-LEUKEMIA
Autore:
GREENBERG SJ; CHOI Y; BALLOW M; DU TL; WARD PM; RICKERT MH; FRANKEL S; BERNSTEIN SH; BRECHER ML;
Indirizzi:
ROSWELL PK CANC INST,DEPT NEUROL,NEUROIMMUNOL & NEUROVIROL LAB,ELM & CARLTON ST BUFFALO NY 14263 ROSWELL PK CANC INST,DEPT MED BUFFALO NY 14263 ROSWELL PK CANC INST,DEPT PEDIAT BUFFALO NY 14263 ROSWELL PK CANC INST,DEPT MOLEC DIAGNOST BUFFALO NY 14263 ROSWELL PK CANC INST,DEPT MICROBIOL & IMMUNOL BUFFALO NY 14263 SUNY BUFFALO,DEPT NEUROL BUFFALO NY 00000 SUNY BUFFALO,DEPT PEDIAT BUFFALO NY 00000 SUNY BUFFALO,DIV ALLERGY & IMMUNOL BUFFALO NY 00000 CHILDRENS HOSP BUFFALO BUFFALO NY 00000
Titolo Testata:
Journal of leukocyte biology
fascicolo: 6, volume: 57, anno: 1995,
pagine: 856 - 864
SICI:
0741-5400(1995)57:6<856:POIHVG>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MINIMAL RESIDUAL DISEASE; RECEPTOR DELTA-CHAIN; B-LYMPHOID CLONALITY; T-CELL; LYMPHOPROLIFERATIVE DISORDERS; REGION GENE; OLIGONUCLEOTIDE PROBES; INVITRO AMPLIFICATION; LYMPHOCYTIC-LEUKEMIA; ANTIBODY REPERTOIRE;
Keywords:
ACUTE LYMPHOBLASTIC LEUKEMIA; B-ALL; GENE REARRANGEMENT; IMMUNOGLOBULIN HEAVY CHAIN; B CELL; POLYMERASE CHAIN REACTION; GENE AMPLIFICATION; MOLECULAR PROBES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
S.J. Greenberg et al., "PROFILE OF IMMUNOGLOBULIN HEAVY-CHAIN VARIABLE GENE REPERTOIRES AND HIGHLY SELECTIVE DETECTION OF MALIGNANT CLONOTYPES IN ACUTE LYMPHOBLASTIC-LEUKEMIA", Journal of leukocyte biology, 57(6), 1995, pp. 856-864

Abstract

The predominant B cell immunoglobulin heavy chain variable gene (IgH-V) usage and the uniquely rearranged, clonotype-specific variable-diversity-joining region gene (VDJ) sequences were identified in patients with B cell acute lymphoblastic leukemia (B-ALL) using a novel DNA-based gene amplification strategy, The approach allows a thorough and sensitive determination of the number of clonal leukemic IgH rearrangements and their precise V gene usage, This strategy may be applied in thedetection of minimal residual disease, in surveillance after induction of disease-free states, and in analyzing the effectiveness of purging autologous bone marrow of malignant clones, An initial primary polymerase chain reaction (PCR), directed by an IgH-J generic primer and a complement of family-specific IgH-V primers, defined the major B cell IgH-V gene usage, Use of an IgH-J generic primer supplanted the use ofa constant region primer anchor and thus eliminated the need to target mRNA by the traditional RNA reverse transcription-PCR amplification method, Monoclonality of rearranged VDJ bands was further substantiated by high resolution denaturant gel electrophoretic analysis, The predominant amplified bands were subcloned and sequenced, By sequencing through VDJ juxtaposed regions, that is, the third complementarity-determining region, clonotype-specific primers were developed and used in asecondary clonotype primer-directed PCR (CPD-PCR) to detect, with extreme sensitivity and specificity, a unique B cell clone, Analysis of the products of the CPD-PCR permitted the detection of a single malignant cell among 1 million polyclonal cells and superseded the constraints of prior studies that have provided a limited evaluation of family variable gene repertoire usage, Leukemic clonal rearrangements were detected in 100% of the eight cases of pediatric and two cases of adult B-ALL studied, Two or more clonal IgH-VDJ amplified sequences were observed in 50% of the B-ALL bone marrows analyzed. In two cases, clonotype-specific oligodeoxynucleotide primers, derived from B-ALL VDJ sequences, directed the secondary CPD-PCR, and disease activity was monitored after chemotherapy and allogeneic bone marrow transplantation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:08:51