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Titolo:
MHV-A59 ENTERS POLARIZED MURINE EPITHELIAL-CELLS THROUGH THE APICAL SURFACE BUT IS RELEASED BASOLATERALLY
Autore:
ROSSEN JWA; VOORHOUT WF; HORZINEK MC; VANDERENDE A; STROUS GJAM; ROTTIER PJM;
Indirizzi:
UNIV UTRECHT,INST VIROL,DEPT IMMUNOL & INFECT DIS,YALELAAN 1 3584 CL UTRECHT NETHERLANDS UNIV UTRECHT,INST VIROL,DEPT IMMUNOL & INFECT DIS 3584 CL UTRECHT NETHERLANDS UNIV UTRECHT,FAC VET MED,DEPT FUNCT MORPHOL 3584 CL UTRECHT NETHERLANDS UNIV UTRECHT,SCH MED,CELL BIOL LAB 3584 CX UTRECHT NETHERLANDS
Titolo Testata:
Virology
fascicolo: 1, volume: 210, anno: 1995,
pagine: 54 - 66
SICI:
0042-6822(1995)210:1<54:MEPMET>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE HEPATITIS-VIRUS; THICK ASCENDING LIMB; MDCK CELLS; HIPPOCAMPAL-NEURONS; SIMIAN VIRUS-40; CANCER-CELLS; SENDAI VIRUS; 2 STRAINS; CORONAVIRUS; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
J.W.A. Rossen et al., "MHV-A59 ENTERS POLARIZED MURINE EPITHELIAL-CELLS THROUGH THE APICAL SURFACE BUT IS RELEASED BASOLATERALLY", Virology, 210(1), 1995, pp. 54-66

Abstract

Coronaviruses have a marked tropism for epithelial cells. Entry and release of the porcine transmissible gastroenteritis virus (TGEV) is restricted to apical surfaces of polarized epithelial cells, as we have recently shown (J. W. A. Rossen, C. P. J. Bekker, W. F. Voorhout, G. J. A. M. Strous, A van der Ende, and P. J. M. Rottier, 1994, J. Virol. 68, 7966-7973). In this paper we analyze the interactions of mouse hepatitis coronavirus A59 (MHV-A59) with polarized murine kidney cells (mTAL) grown on permeable supports. After inoculation from the apical orbasolateral side, virus entry was found to take place only through the apical membrane. The virus utilized a protein of the carcinoembryonic antigen family as its receptor. In contrast to TGEV, MHV-A59 was released preferentially from the basolateral plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the basolateral culture fluid as well as by electron microscopical observations. In the mouse, MHV initially replicates in the nasal epithelium before being disseminated throughout the body; the basolateral releaseof MHV from epithelial cells into the animal's circulation may be thefirst step in the establishment of a systemic infection. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:50:13