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Titolo:
ROTAVIRUS SUBUNIT VACCINES
Autore:
CONNER ME; CRAWFORD SE; BARONE C; ONEAL C; ZHOU YJ; FERNANDEZ F; PARWANI A; SAIF LJ; COHEN J; ESTES MK;
Indirizzi:
BAYLOR COLL MED,DIV MOL VIROL,1 BAYLOR PLAZA HOUSTON TX 77030 VET ADM MED CTR HOUSTON TX 77211 OHIO STATE UNIV,OHIO AGR RES & DEV CTR WOOSTER OH 44691 INRA,UNIV VIROL & IMMUNOL MOL JOUY EN JOSAS FRANCE
Titolo Testata:
Archives of virology
, , anno: 1996, supplemento:, 12
pagine: 199 - 206
SICI:
0304-8608(1996):<199:RSV>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNE-RESPONSE; RABBIT MODEL; EXPRESSION; INFECTION; PARTICLES; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M.E. Conner et al., "ROTAVIRUS SUBUNIT VACCINES", Archives of virology, 1996, pp. 199-206

Abstract

We evaluated rotavirus subunit vaccines for use in humans and animals. Insect cells were co-infected with combinations of individual baculovirus recombinants expressing human, bovine or simian rotavirus VP2, VP4, VP6 or VP7 to produce virus-like particles (VLPs). To determine whether immunization with VLPs could induce active protective immunity, VLPs were administered parenterally to rabbits, and the immune response and protection from rabbit ALA rotavirus challenge were evaluated. Complete or partial protection was attained, showing that parenteral immunization with VLPs induces active protective immunity. We also examined whether heterotypic immune responses could be induced with a limited number of broadly reactive VP7 proteins or with chimeric particles (multiple VP7 types on individual particles). The feasibility of this approach was determined by immunizing mice with VLPs containing a G3 VP7 or G1 VP7 and chimeric G1/G3 VLPs. Broadly reactive neutralizing antibody was induced by the G1 VLPs. VLPs also have been successfully used to boost lactogenic (colostral and milk) immunity in dairy cows. Taken together, these results show that VLPs can be effective immunogensin rabbits, mice and dairy cattle when administered parenterally, a limited number of VLPs may be sufficient to produce a broadly protective vaccine, and G3 VLPs may serve as an effective subunit vaccine for use in bovines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 13:52:19