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Titolo:
HAPTEN SYNTHESIS AND DEVELOPMENT OF ELISAS FOR DETECTION OF ENDOSULFAN IN WATER AND SOIL
Autore:
LEE NJ; SKERRITT JH; MCADAM DP;
Indirizzi:
CSIRO,DIV PLANT IND,GPO BOX 1600 CANBERRA ACT 2601 AUSTRALIA CSIRO,DIV PLANT IND CANBERRA ACT 2601 AUSTRALIA CSIRO,DIV PLANT IND N RYDE NSW 2113 AUSTRALIA
Titolo Testata:
Journal of agricultural and food chemistry
fascicolo: 6, volume: 43, anno: 1995,
pagine: 1730 - 1739
SICI:
0021-8561(1995)43:6<1730:HSADOE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-ENDOSULFAN; BETA-ENDOSULFAN; RESIDUES; IMMUNOASSAY; FISH; CHROMATOGRAPHY; INSECTICIDES; PESTICIDES; PRODUCTS;
Keywords:
IMMUNOASSAY; ENDOSULFAN; ORGANOCHLORINE; INSECTICIDE; FIELD TEST; ENVIRONMENTAL ANALYSIS; SOIL WATER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
N.J. Lee et al., "HAPTEN SYNTHESIS AND DEVELOPMENT OF ELISAS FOR DETECTION OF ENDOSULFAN IN WATER AND SOIL", Journal of agricultural and food chemistry, 43(6), 1995, pp. 1730-1739

Abstract

Two enzyme immunoassays, a laboratory assay based on microwell platesand a field test based on the use of small polystyrene tubes, have been developed for the detection of endosulfan exachloro-8,9,10-trinorborn-5-en-2,3-ylenedimethyl sulfite) residues in water and soil. To raise antibodies that are sensitive and selective for the toxic forms of endosulfan, three haptens were prepared. One hapten was prepared by derivatization of endosulfan diol 7-hexachloro-2,3-bis(hydroxymethyl)norborn-5-ene], while the others used derivatives of a rigid five-memberedring adjacent to the bridged hexachlorocyclopentadiene (cyclodiene) ring. Different hapten combinations were used for immunogen and reporter enzyme conjugate in both the microwell and field assays. The optimized assays have detection limits of about 0.2 ppb endosulfan and detectin the range 0.2-10 ppb (0.2-20 ppb for field assay, without sample dilution). Water samples can be analyzed directly without solvent extraction or concentration, while soil samples are simply extracted with 90% methanol. The tests detect endosulfan sulfate exachloro-8,9,10-trinorborn-5-en-2,3-ylenedimethyl sulfate) with sensitivity similar to that for endosulfan but-are 4-10 times less sensitive to endosulfan diol,and therefore the assays can potentially determine toxic compounds ofendosulfan (endosulfan and endosulfan sulfate) from the total endosulfan residues present in the environment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 12:16:04