Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CLONING OF A RECEPTOR FOR AMPHIBIAN [PHE(13)]BOMBESIN DISTINCT FROM THE RECEPTOR FOR GASTRIN-RELEASING PEPTIDE - IDENTIFICATION OF A 4TH BOMBESIN RECEPTOR SUBTYPE (BB4)
Autore:
NAGALLA SR; BARRY BJ; CRESWICK KC; EDEN P; TAYLOR JT; SPINDEL ER;
Indirizzi:
OREGON REG PRIMATE RES CTR,DIV NEUROSCI,505 NW 185TH AVE BEAVERTON OR97006 BIOMEASURE INC MILFORD MA 01757
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 13, volume: 92, anno: 1995,
pagine: 6205 - 6209
SICI:
0027-8424(1995)92:13<6205:COARFA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUNG-CARCINOMA CELLS; SWISS 3T3 CELLS; MOLECULAR-CLONING; MAMMALIAN BOMBESIN; NEUROMEDIN-B; EXPRESSION; INHIBITION; BOMBINA; BINDING; POTENT;
Keywords:
BOMBINA ORIENTALIS; FROG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
S.R. Nagalla et al., "CLONING OF A RECEPTOR FOR AMPHIBIAN [PHE(13)]BOMBESIN DISTINCT FROM THE RECEPTOR FOR GASTRIN-RELEASING PEPTIDE - IDENTIFICATION OF A 4TH BOMBESIN RECEPTOR SUBTYPE (BB4)", Proceedings of the National Academy of Sciences of the United Statesof America, 92(13), 1995, pp. 6205-6209

Abstract

Bombesin is a tetradecapeptide originally isolated from frog skin anddemonstrated to have a wide range of actions in mammals, Based on structural homology and similar biological activities, gastrin-releasing peptide (GRP) has been considered the mammalian equivalent of bombesin, We previously reported that frogs have both GRP and bombesin, which therefore are distinct peptides, We now report the cloning of a bombesin receptor subtype (BB4) that has higher affinity for bombesin than GRP. PCR was used to amplify cDNAs related to the known bombesin receptors from frog brain, Sequence analysis of the amplified cDNAs revealed3 classes of receptor subtypes, Based on amino acid homology, two classes were clearly the amphibian homologs of the GRP and neuromedin B receptors, The third class was unusual and a full-length clone was isolated from a Bombina orientalis brain cDNA library. Expression of the receptor in Xenopus oocytes demonstrated that the receptor responded topicomolar concentrations of [Phe(13)]bombesin, the form of bombesin most prevalent in frog brain, The relative rank potency of bombesin like peptides for this receptor was [Phe(13)]bombesin > [Leu(13)]bombesin> GRP > neuromedin B. In contrast, the rank potency for the GRP receptor is GRP > [Leu(13)]bombesin > [Phe(13)]bombesin > neuromedin B. Transient expression in CHOP cells gave a K-i for [Phe(13)]bombesin of 0.2 nM versus a K-i of 2.1 nM for GRP, Distribution analysis showed thatthis receptor was expressed only in brain, consistent with the distribution of [Phe(13)]bombesin, Thus, based on distribution and affinity,this bombesin receptor is the receptor for [Phe(13)]bombesin. Phylogenetic analysis suggests that this receptor separated prior to separation of the GRP and neuromedin B receptors; thus, BB4 receptors and their cognate ligands may also exist in mammals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:20:59