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Titolo:
EXPRESSION CLONING OF A CDNA-ENCODING A FISH PROLACTIN RECEPTOR
Autore:
SANDRA O; SOHM F; DELUZE A; PRUNET P; EDERY M; KELLY PA;
Indirizzi:
FAC MED NECKER ENFANTS MALAD,INSERM,U344 F-75730 PARIS 15 FRANCE FAC MED NECKER ENFANTS MALAD,INSERM,U344 F-75730 PARIS 15 FRANCE INRA,PHYSIOL POISSONS LAB F-35042 RENNES FRANCE MUSEUM NATL HIST NAT,COMPAREE PHYSIOL LAB,CNRS,URA 90 F-75231 PARIS 05 FRANCE
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 13, volume: 92, anno: 1995,
pagine: 6037 - 6041
SICI:
0027-8424(1995)92:13<6037:ECOACA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
TILAPIA OREOCHROMIS-MOSSAMBICUS; GROWTH-HORMONE; MOLECULAR-CLONING; OVINE PROLACTIN; BINDING DOMAIN; GENE; PURIFICATION; SEQUENCE; TISSUES; FAMILY;
Keywords:
TILAPIA; KIDNEY; OSMOREGULATION; CONSERVED RECEPTOR DOMAINS; MAMMALIAN CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
O. Sandra et al., "EXPRESSION CLONING OF A CDNA-ENCODING A FISH PROLACTIN RECEPTOR", Proceedings of the National Academy of Sciences of the United Statesof America, 92(13), 1995, pp. 6037-6041

Abstract

By using an expression cloning strategy, we isolated a single positive clone encoding a tilapia prolactin (PRL) receptor, Tilapia PRL(188) was used to screen a freshwater tilapia kidney expression library transfected in COS cells, The tilapia PRL receptor is a mature protein of 606 amino acids, The extracellular domain is devoid of the tandem repeat units present in birds and has two pairs of cysteine residues, a Trp-Ser-Xaa-Trp-Ser motif, and two potential N-glycosylation sites, The cytoplasmic domain contains 372 amino acids, including box 1, a sequence previously shown to be important for signal transduction in mammalian species, Thus, the general structure is similar to the long form ofmammalian PRL receptors; however, amino acid comparisons reveal a rather low identity (approximate to 37%). Northern blot analysis shows the existence of a single transcript in osmoregulatory tissues and reproductive organs, This localization is in agreement with known functionsof PRL in teleosts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:39:51