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Titolo:
DEPENDENCE OF ECDYSTEROID METABOLISM AND DEVELOPMENT IN HOST LARVAE ON THE TIME OF BACULOVIRUS INFECTION AND THE ACTIVITY OF THE UDP-GLUCOSYL TRANSFERASE GENE
Autore:
BURAND JP; PARK EJ; KELLY TJ;
Indirizzi:
UNIV MASSACHUSETTS,DEPT MICROBIOL AMHERST MA 01003 UNIV MASSACHUSETTS,DEPT ENTOMOL AMHERST MA 01003 USDA ARS,BELTSVILLE AGR RES CTR,INSECT NEUROBIOL & HORMONE LAB,PSI BELTSVILLE MD 20705
Titolo Testata:
Insect biochemistry and molecular biology
fascicolo: 8-9, volume: 26, anno: 1996,
pagine: 845 - 852
SICI:
0965-1748(1996)26:8-9<845:DOEMAD>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GYPSY-MOTH LARVAE; LYMANTRIA-DISPAR; PROTHORACICOTROPIC HORMONE; FEEDBACK INHIBITION; ECDYSONE PRODUCTION; 20-HYDROXYECDYSONE;
Keywords:
BACULOVIRUS; LYMANTRIA DISPAR; NUCLEAR POLYHEDROSIS VIRUS; LDNPV; ECDYSTEROID; ECDYSTEROID; UDP-GLUCOSYL TRANSFERASE; PROTHORACIC GLAND;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
J.P. Burand et al., "DEPENDENCE OF ECDYSTEROID METABOLISM AND DEVELOPMENT IN HOST LARVAE ON THE TIME OF BACULOVIRUS INFECTION AND THE ACTIVITY OF THE UDP-GLUCOSYL TRANSFERASE GENE", Insect biochemistry and molecular biology, 26(8-9), 1996, pp. 845-852

Abstract

Infection of fourth-instar gypsy moth (Lymantria dispar, Lepidoptera:Lymantriidae) larvae with the wild-type (Wt) gypsy moth baculovirus, LdNPV on the first day post-molt, or infection of fifth instars on thefifth day post-molt, results in elevated ecdysteroid levels in both hemolymph and the media of cultured prothoracic glands, when examined by radioimmunoassay and compared to mock-infected controls, In contrast, insects infected with an ecdysteroid UDP-glucosyl transferase minus [egt(-)] mutant virus show ecdysteroid levels and gland activity similar to that found in controls, UDP-glucosyl transferase causes sugar-conjugation of ecdysteroids containing a hydroxyl group at the C-22 position, and our ecdysteroid antiserum recognizes these conjugated ecdysteroids, Since we found that 20-hydroxyecdysone inhibits gland activityin culture and glucose-conjugated ecdysone, the major form of ecdysteroid in the hemolymph of Wt baculovirus-infected animals does not, theelevated level of hemolymph ecdysteroids and heightened gland activity in Wt virus-infected animals are most likely due to low levels of 20-hydroxyecdysone resulting from sugar conjugation of ecdysone by EGT, Interestingly, hemolymph ecdysteroid titer and gland activity of fifth-instar larvae infected with either the Wt or egt(-) virus on the first day post-molt remain at basal levels throughout the instar, with death occurring prior to pupation, suggesting that LdNPV may have a second, egt-independent mechanism for regulation of host ecdysteroid levels. Copyright (C) 1996 Elsevier Science Ltd

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Documento generato il 21/09/20 alle ore 04:37:11