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Titolo:
DISTRIBUTION OF DEVELOPMENTALLY-REGULATED TRANS-SIALIDASES IN THE KINETOPLASTIDA AND CHARACTERIZATION OF A SHED TRANS-SIALIDASE ACTIVITY FROM PROCYCLIC TRYPANOSOMA-CONGOLENSE
Autore:
ENGSTLER M; SCHAUER R; BRUN R;
Indirizzi:
CHRISTIAN ALBRECHTS UNIV KIEL,INST BIOCHEM,OLSHAUSENSTR 40 D-24098 KIEL GERMANY CHRISTIAN ALBRECHTS UNIV KIEL,INST BIOCHEM D-24098 KIEL GERMANY SWISS TROP INST BASEL SWITZERLAND
Titolo Testata:
Acta Tropica
fascicolo: 2, volume: 59, anno: 1995,
pagine: 117 - 129
SICI:
0001-706X(1995)59:2<117:DODTIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACIDIC REPETITIVE PROTEIN; AFRICAN TRYPANOSOMES; CULTURE FORMS; CRUZI; BRUCEI; CULTIVATION; MEMBRANE; IDENTIFICATION; GLYCOPROTEINS; NEURAMINIDASE;
Keywords:
TRANS-SIALIDASE; SIALIDASE; KINETOPLASTIDA; TRYPANOSOMA CONGOLENSE; TRYPANOSOMA VIVAX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
M. Engstler et al., "DISTRIBUTION OF DEVELOPMENTALLY-REGULATED TRANS-SIALIDASES IN THE KINETOPLASTIDA AND CHARACTERIZATION OF A SHED TRANS-SIALIDASE ACTIVITY FROM PROCYCLIC TRYPANOSOMA-CONGOLENSE", Acta Tropica, 59(2), 1995, pp. 117-129

Abstract

The expression of developmentally regulated sialidase and trans-sialidase activities in kinetoplastid protozoa was investigated. The occurrence of these enzymes was found not to be a common feature among the Kinetoplastida, but to be restricted to distinct developmental life cycle stages of only a few species. While sialidases without trans-sialylating activities were demonstrated in Trypanosoma vivax and T. rangeli, trans-sialidase activity is expressed throughout the brucei-group and in T. congolense. Neither T. evansi, nor T. equiperdum express sialidases or trans-sialidases. Furthermore, the absence of both, sialidaseand trans-sialidase activities was proven in the Leishmania, Crithidia, Herpetomonas, Leptomonas and Phytomonas, respectively. In all species tested, the occurrence of sialic acids coincides with the expression of trans-sialidase activity. Those parasites, which lack trans-sialidases or only display regular sialidases, also lack cell-bound sialic acids. The regular sialidase activity from bloodstream form T. vivax was characterized. The trans-sialidase from T. congolense is restrictedto the procyclic culture forms and is shed into the culture medium. The enzyme has a pH-optimum at pH 7.0, displays sensitivity towards chlorides and is resistant against commonly used sialidase inhibitors. T.congolense trans-sialidase transfers preferentially alpha(2-3)-linkedsialic acids onto terminal beta-galactose residues. Also hydroxylatedsialic acids (Neu5Gc) are transferred. The major glycoprotein GARP from procyclic T. congolense was identified as one potential natural sialic acid acceptor on the parasite's surface. In order to facilitate the characterization of trans-sialidases a novel, fluorimetric trans-sialidase assay was developed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 01:29:36