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Titolo:
PHORBOL ESTER (12-O-TETRADECANOYLPHORBOL 13-ACETATE) PREVENTS ORNITHINE DECARBOXYLASE INHIBITION AND APOPTOSIS IN L1210 LEUKEMIC-CELLS EXPOSED TO TGF-BETA(1)
Autore:
MOTYL T; KASTERKA M; GRZELKOWSKA K; OSTROWSKI J; FILIPECKI M; MALICKA E; PLOSZAJ T;
Indirizzi:
AGR UNIV WARSAW,FAC VET MED,DEPT ANIM PHYSIOL,NOWOURSYNOWSKA 166 PL-02787 WARSAW POLAND MED CTR POSTGRAD EDUC,DEPT GASTROENTEROL WARSAW POLAND AGR UNIV WARSAW,FAC HORT,DEPT PLANT GENET & BREEDING HORT WARSAW POLAND AGR UNIV WARSAW,FAC VET MED,DEPT PATHOL WARSAW POLAND
Titolo Testata:
International journal of biochemistry & cell biology
fascicolo: 12, volume: 28, anno: 1996,
pagine: 1327 - 1335
SICI:
1357-2725(1996)28:12<1327:PE(1PO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; PROTEIN-KINASE-C; HUMAN NEUROBLASTOMA-CELLS; TRANSFORMING GROWTH-FACTOR-BETA-1; TISSUE TRANSGLUTAMINASE; TUMOR PROGRESSION; MYC EXPRESSION; HEPATOMA-CELLS; RETINOIC ACID; CANCER CELLS;
Keywords:
TGF-BETA(1); APOPTOSIS; ORNITHINE DECARBOXYLASE; POLYAMINES; PHORBOL ESTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
T. Motyl et al., "PHORBOL ESTER (12-O-TETRADECANOYLPHORBOL 13-ACETATE) PREVENTS ORNITHINE DECARBOXYLASE INHIBITION AND APOPTOSIS IN L1210 LEUKEMIC-CELLS EXPOSED TO TGF-BETA(1)", International journal of biochemistry & cell biology, 28(12), 1996, pp. 1327-1335

Abstract

Previous studies have shown that growth suppression and apoptosis of leukemic cells exposed to TGF-beta(1) is associated with the inhibition of ornithine decarboxylase (ODC) - the key enzyme of polyamine pathway. The aim of the present study was to evaluate the influence of 12-O-tetradecanoylphorbol 13-acetate (TPA) - a potent ODC inducer on antiproliferative and apoptotic effects of TGF-beta(1) in L1210 leukemic cells. Cells were incubated in 2%FCS/RPMI-1640 medium, supplemented withTGF-beta(1) (2 ng/ml), TPA (100 ng/ml) or alpha-difluoromethylornithine (DFMO) (5 mM). Cell proliferation, apoptosis and necrosis were evaluated using [methyl-H-3] thymidine, electron microscopy, electrophoresis of DNA and trypan blue exclusion. Expression and activity of ODC were determinated by RT-PCR and measurement of (CO2)-C-14 release from L-1-C-14 ornithine, respectively. TGF-beta(1) inhibited proliferation and induced apoptotic and necrotic cell death in L1210 leukemic cells. The above effects were associated with the inhibition of ODC expression and activity, measured 2 and 4 hr after TGF-beta(1) administration, respectively. The presence of DEMO, an irreversible inhibitor of ODC, led to apoptotic fragmentation of DNA, similar to that observed in TGF-beta(1)-treated cultures. Administration of TPA simultaneously with TGF-beta(1) significantly reduced antiproliferative, apoptotic and necrotic effects of TGF-beta(1), and prevented its inhibitory action on ODC expression and activity. It is concluded that: down-regulation of ODC expression may be one of the early events associated with TGF-beta(1)-evoked suppression of growth and apoptosis; ODC is involved in the mechanism of protective action of TPA on TGF-beta(1)-related growth inhibition of L1210 leukemic cells. Copyright (C) 1996 Elsevier Science Ltd

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 09:25:11