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Titolo:
BIOSYNTHESIS OF PLATELET-ACTIVATING-FACTOR IN NORMAL AND INFLAMED HUMAN COLON MUCOSA - EVIDENCE FOR THE INVOLVEMENT OF THE PATHWAY OF PLATELET-ACTIVATING-FACTOR SYNTHESIS DE-NOVO IN INFLAMMATORY BOWEL-DISEASE
Autore:
APPLEYARD CB; HILLIER K;
Indirizzi:
UNIV SOUTHAMPTON,FAC MED,CLIN PHARMACOL GRP,BIOMED SCI BLDG,BASSETT CRESCENT E SOUTHAMPTON SO16 7PX HANTS ENGLAND UNIV SOUTHAMPTON,FAC MED,CLIN PHARMACOL GRP SOUTHAMPTON SO16 7PX HANTS ENGLAND
Titolo Testata:
Clinical science
fascicolo: 6, volume: 88, anno: 1995,
pagine: 713 - 717
SICI:
0143-5221(1995)88:6<713:BOPINA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
RABBIT PLATELETS; GASTRIC-MUCOSA; CROHNS-DISEASE; METABOLISM; 1-ALKYL-2-ACETYL-SN-GLYCERO-3-PHOSPHOCHOLINE;
Keywords:
INFLAMMATORY BOWEL DISEASE; PLATELET-ACTIVATING FACTOR; SYNTHESIS DE NOVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
C.B. Appleyard e K. Hillier, "BIOSYNTHESIS OF PLATELET-ACTIVATING-FACTOR IN NORMAL AND INFLAMED HUMAN COLON MUCOSA - EVIDENCE FOR THE INVOLVEMENT OF THE PATHWAY OF PLATELET-ACTIVATING-FACTOR SYNTHESIS DE-NOVO IN INFLAMMATORY BOWEL-DISEASE", Clinical science, 88(6), 1995, pp. 713-717

Abstract

1. Platelet-activating factor can be synthesized by two distinct biochemical pathways and is degraded by a number of enzymes, the first step of which is deacetylation by a specific acetyl hydrolase. 2. The biochemical pathway of platelet-activating factor synthesis de novo and the first step in platelet-activating factor degradation have been investigated for the first time in incubates of normal human colon mucosa and in inflamed mucosa from patients with inflammatory bowel disease. 3. In the presence of 100 mu mol/l CDP-choline and 100 mu mol/l hexadecyl acetyl glycerol, homogenates from inflamed mucosa synthesized significantly greater platelet-activating factor [851 +/- 574 pmol/mg of protein (mean +/- SEM) in 90 min incubation] than normal mucosa [105 +/- 61 pmol/mg of protein in 90 min incubation] (P < 0,05). 4. Under thesame conditions of assay, the percentage turnover to inactive lyso-platelet-activating factor was similar in inflamed mucosa (35.5 +/- 9.4%) and normal mucosa (42.7 +/- 8.5%) in 90 min (P > 0.05). 5. The identity of platelet-activating factor was confirmed by HPLC, by its mobility on TLC and by the ability of WEB 2170, a selective platelet-activating factor receptor antagonist, to block its platelet-aggregatory action. 6. These findings confirm the presence of the pathway for the synthesis de novo of the potently proinflammatory platelet-activating factor in human colon mucosa in inflammatory bowel disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 01:47:24