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Titolo:
EXPRESSION OF INSULIN-LIKE GROWTH-FACTOR-1 (IGF-1) IN THE BOVINE OVIDUCT DURING THE ESTROUS-CYCLE
Autore:
SCHMIDT A; EINSPANIER R; AMSELGRUBER W; SINOWATZ F; SCHAMS D;
Indirizzi:
FORSCHUNGSZENTRUM MILCH & LEBENSMITTEL,INST PHYSIOL,VOTTINGERSTR 45 D-85350 FREISING GERMANY LMU,INST ANAT 2 MUNICH GERMANY TECH UNIV MUNICH,INST PHYSIOL D-85350 FREISING GERMANY
Titolo Testata:
Experimental and clinical endocrinology
fascicolo: 5, volume: 102, anno: 1994,
pagine: 364 - 369
SICI:
0232-7384(1994)102:5<364:EOIG(I>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-I; CO-CULTURE; PROTEIN-SYNTHESIS; ESTROUS-CYCLE; FLUID; IDENTIFICATION; RECEPTOR; INVITRO; EMBRYOS; ESTRUS;
Keywords:
IGF-1; MESSENGER-RNA; CATTLE; OVIDUCT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
A. Schmidt et al., "EXPRESSION OF INSULIN-LIKE GROWTH-FACTOR-1 (IGF-1) IN THE BOVINE OVIDUCT DURING THE ESTROUS-CYCLE", Experimental and clinical endocrinology, 102(5), 1994, pp. 364-369

Abstract

Growth factors are known to take part in the regulation of reproduction. Here we present evidence for the expression of insulin-like growthfactor 1 (IGF-1) in the bovine oviduct. Two IGF-1 specific mRNA-transcripts of 1.5 and 4.4 kb were detected during the whole oestrous cycle, acid showed increased expression after ovulation. Complete homology to the known IGF-1 sequence was achieved by specific RT-PCR (reverse transcription - polymerase chain reaction) amplification followed by dideoxysequencing of this 210 bp fragment. IGF-1 protein was localized in the secretory cells of the oviduct epithelium using immunohistochemical techniques. We suggest possible effects of IGF-1 during ovulation either on the oviductal cells or on the early embryo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:18:12