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Titolo:
PSORALEN TREATMENT OF ADENOVIRUS PARTICLES ELIMINATES VIRUS-REPLICATION AND TRANSCRIPTION WHILE MAINTAINING THE ENDOSOMOLYTIC ACTIVITY OF THE VIRUS CAPSID
Autore:
COTTEN M; SALTIK M; KURSA M; WAGNER E; MAASS G; BIRNSTIEL ML;
Indirizzi:
RES INST MOLEC PATHOL,DR BOHR GASSE 7 A-1030 VIENNA AUSTRIA BENDER A-1121 VIENNA AUSTRIA
Titolo Testata:
Virology
fascicolo: 1, volume: 205, anno: 1994,
pagine: 254 - 261
SICI:
0042-6822(1994)205:1<254:PTOAPE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE DELIVERY; PRIMARY HEPATOCYTES; BETA-PROPIOLACTONE; CELL-INTERACTIONS; MAMMALIAN-CELLS; EARLY REGION-4; DNA; EXPRESSION; INACTIVATION; POLYLYSINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
M. Cotten et al., "PSORALEN TREATMENT OF ADENOVIRUS PARTICLES ELIMINATES VIRUS-REPLICATION AND TRANSCRIPTION WHILE MAINTAINING THE ENDOSOMOLYTIC ACTIVITY OF THE VIRUS CAPSID", Virology, 205(1), 1994, pp. 254-261

Abstract

Adenovirus entry into its host cell transiently permeabilizes the cell allowing the coentry of reagents such as DNA. We compare here adenovirus inactivation with beta-propiolactone and several psoralen derivatives, seeking reagents that disrupt the viral genome without impairingthe viral entry functions. No virus replication can be detected after8-methoxypsoralen (8-MOP) modification. Viral transcription is not detectable by Northern analysis, and reverse transcriptase/PCR analysis demonstrates at least a 1000-fold decrease in viral transcription after 8-MOP treatment. Using [H-3]8-MOP, the psoralen is found to enter the virus capsid and react throughout the viral genome, with approximately one psoralen modification per 100 bp of viral DNA. This inactivatedadenovirus allows us to deliver DNA to target cells without interference from adenovirus gene expression or replication. Furthermore, we can now study the host cell response to adenovirus entry without the complications of adenovirus gene expression. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:30:34