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Titolo:
ECTOENZYME REGULATION BY PHENOTYPICALLY DISTINCT FIBROBLAST SUBPOPULATIONS ISOLATED FROM THE HUMAN MAMMARY-GLAND
Autore:
ATHERTON AJ; OHARE MJ; BULUWELA L; TITLEY J; MONAGHAN P; PATERSON HF; WARBURTON MJ; GUSTERSON BA;
Indirizzi:
INST CANC RES,HADDOW LABS,15 COTSWOLD RD SUTTON SM2 5NG SURREY ENGLAND INST CANC RES,HADDOW LABS SUTTON SM2 5NG SURREY ENGLAND CHARING CROSS & WESTMINSTER MED SCH,DEPT BIOCHEM LONDON W6 8RF ENGLAND INST CANC RES,CHESTER BEATTY LABS LONDON SW3 6JB ENGLAND ST GEORGE HOSP,SCH MED,DEPT HISTOPATHOL LONDON SW17 0RE ENGLAND
Titolo Testata:
Journal of Cell Science
, volume: 107, anno: 1994,
parte:, 10
pagine: 2931 - 2939
SICI:
0021-9533(1994)107:<2931:ERBPDF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUTRAL ENDOPEPTIDASE 24.11; DIPEPTIDYL PEPTIDASE-IV; LEUKEMIA ANTIGEN; MYOEPITHELIAL CELLS; AMINOPEPTIDASE-N; EPITHELIAL-CELLS; SUBSTANCE-P; EXPRESSION; ENKEPHALINASE; DIFFERENTIATION;
Keywords:
FIBROBLAST; HUMAN BREAST; ECTOENZYME; NEUTRAL ENDOPEPTIDASE; CD10; AMINOPEPTIDASE N; CD13; DIPEPTIDYL PEPTIDASE IV; CD26;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Atherton et al., "ECTOENZYME REGULATION BY PHENOTYPICALLY DISTINCT FIBROBLAST SUBPOPULATIONS ISOLATED FROM THE HUMAN MAMMARY-GLAND", Journal of Cell Science, 107, 1994, pp. 2931-2939

Abstract

Inter- and intralobular mammary fibroblasts have been separated from normal human breast tissue and cultured to study the differential expression of ectoenzymes present within the stroma of the normal gland and associated with breast cancers. Specific ectoenzymes were identifiedby indirect immunofluorescence and quantified by flow cytometry and semi-quantitative PCR. A consistent difference was noted between the two fibroblast sub-populations at early passage in respect of dipeptidylpeptidase IV (DPP IV) and aminopeptidase N (APN) expression. Early passage intralobular fibroblasts were positive for APN but negative for DPP IV, as seen in the intact tissue. However, with continued sub-culture they gradually began to express DPP TV, until at later passages they became indistinguishable from the interlobular fibroblasts, which were APN and DPP IV-positive at all stages in culture, as they are in intact tissue. Neutral endopeptidase (NEP/CALLA/CD10) is not expressed by normal adult breast fibroblasts but is found in the stroma associated with over 60% of breast cancers. It was up-regulated in vitro on both inter- and intralobular fibroblasts, with final levels that were significantly (<14 times) higher on the former in all pairs of preparations from individual donors analysed. This difference persisted with continued passage, and levels of the ectoenzyme and its messenger RNA were further up-regulated by hydrocortisone in both populations. These results demonstrate that phenotypically distinct cultures of human mammary fibroblast sub-populations can be used to study the regulation of these stromal ectoenzymes. Such cultures also provide a means of investigating the mechanisms and functional significance of changes occurring as the result of neoplasia, as well as enabling the individual roles of inter- and intralobular fibroblasts in epithelial/mesenchymal interactions to be evaluated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 09:57:48