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Titolo:
ANTISENSE OLIGONUCLEOTIDES IN SOLUTION OR ENCAPSULATED IN IMMUNOLIPOSOMES INHIBIT REPLICATION OF HIV-1 BY SEVERAL DIFFERENT MECHANISMS
Autore:
ZELPHATI O; IMBACH JL; SIGNORET N; ZON G; RAYNER B; LESERMAN L;
Indirizzi:
CIML,CASE 906 F-13288 MARSEILLE 9 FRANCE CNRS MARSEILLE LUMINY,INSERM,CTR IMMUNOL F-13288 MARSEILLE 9 FRANCE UNIV MONTPELLIER 2,CHIM BIOORGAN LAB,CNRS,URA 488 F-34095 MONTPELLIER5 FRANCE LYNX THERAPEUT INC HAYWARD CA 94545
Titolo Testata:
Nucleic acids research
fascicolo: 20, volume: 22, anno: 1994,
pagine: 4307 - 4314
SICI:
0305-1048(1994)22:20<4307:AOISOE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; CHRONICALLY INFECTED-CELLS; PHOSPHOROTHIOATE ANALOGS; REVERSE TRANSCRIPTION; MESSENGER-RNA; ANOMERIC OLIGODEOXYRIBONUCLEOTIDES; ALPHA-OLIGONUCLEOTIDES; PREMESSENGER RNA; SPLICE JUNCTION; NUCLEIC-ACIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
O. Zelphati et al., "ANTISENSE OLIGONUCLEOTIDES IN SOLUTION OR ENCAPSULATED IN IMMUNOLIPOSOMES INHIBIT REPLICATION OF HIV-1 BY SEVERAL DIFFERENT MECHANISMS", Nucleic acids research, 22(20), 1994, pp. 4307-4314

Abstract

Phosphodiester and phosphorothioate oligonucleotides in alpha and beta configurations directed against the initiation codon region of the HIV-1 rev gene were evaluated for their ability to inhibit HIV-1 replication in acutely and chronically infected human CEM cells. Encapsulation in antibody-targeted liposomes (immunoliposomes) permitted intracellular delivery and distinction between oligonucleotide-mediated inhibition of viral entry and intracellular effects on viral RNA, Our results are consistent with four mechanisms of antiviral activity for these antisense oligonucleotides: (i) interference with virus-mediated cell fusion by free but not liposome-encapsulated phosphorothioate oligonucleotides of any sequence; (ii) interference with reverse transcriptionin a sequence non-specific manner by phosphorothioate oligonucleotides in alpha and beta configurations; (iii) interference with viral reverse transcription in a sequence-specific and RNase-H-independent manner by alpha and beta phosphodiester oligonucleotides; (iv) interferencewith viral mRNA in a sequence-specific and RNase-H-dependent manner by beta-phosphorothioate oligonucleotides.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 15:31:49