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Titolo:
CHARACTERIZATION OF A NOVEL TUMOR-DERIVED CYTOKINE - ENDOTHELIAL-MONOCYTE ACTIVATING POLYPEPTIDE-II
Autore:
KAO J; HOUCK K; FAN Y; HAEHNEL I; LIBUTTI SK; KAYTON ML; GRIKSCHEIT T; CHABOT J; NOWYGROD R; GREENBERG S; KUANG WJ; LEUNG DW; HAYWARD JR; KISIEL W; HEATH M; BRETT J; STERN DM;
Indirizzi:
COLUMBIA UNIV,COLL PHYS & SURG,DEPT PHYSIOL,P&S 11-518,630 W 168TH STNEW YORK NY 10032 COLUMBIA UNIV,COLL PHYS & SURG,DEPT SURG NEW YORK NY 10032 COLUMBIA UNIV,COLL PHYS & SURG,DEPT MED NEW YORK NY 10032 COLUMBIA UNIV,COLL PHYS & SURG,DEPT ANESTHESIA NEW YORK NY 10032 GENENTECH INC,DEPT MOLEC BIOL S SAN FRANCISCO CA 94080 GENENTECH INC,DEPT RECOVERY SCI S SAN FRANCISCO CA 94080 UNIV NEW MEXICO,SCH MED,DEPT PATHOL,BLOOD SYST RES FDN LAB ALBUQUERQUE NM 87131
Titolo Testata:
The Journal of biological chemistry
fascicolo: 40, volume: 269, anno: 1994,
pagine: 25106 - 25119
SICI:
0021-9258(1994)269:40<25106:COANTC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR-PERMEABILITY FACTOR; NEUTROPHIL CHEMOTACTIC FACTOR; NECROSIS-FACTOR CACHECTIN; VONWILLEBRAND ANTIGEN-II; MONONUCLEAR PHAGOCYTES; STIMULATED SECRETION; INTERLEUKIN 1-ALPHA; MOLECULAR-CLONING; MAMMARY-TUMOR; TISSUE FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
74
Recensione:
Indirizzi per estratti:
Citazione:
J. Kao et al., "CHARACTERIZATION OF A NOVEL TUMOR-DERIVED CYTOKINE - ENDOTHELIAL-MONOCYTE ACTIVATING POLYPEPTIDE-II", The Journal of biological chemistry, 269(40), 1994, pp. 25106-25119

Abstract

Endothelial-monocyte activating polypeptide II (EMAP II) was initially identified in the supernatant of murine methylcholanthrene A-inducedfibrosarcomas (Meth A) by its capacity to activate host effector cells (Kao, J., Ryan, J., Brett, J., Chen, J., Shen, H., Fan, Y-G., Godman, G., Familletti, P., Wang, F., Pan, Y-C., Stern, D., and Clauss, M. (1992) J. Biol. Chem. 267, 20239-20247). Based on the NH2-terminal protein sequence, a full-length cDNA has been cloned which indicates that the precursor of EMAP II is a unique, leaderless, single polypeptide chain with predicted molecular mass approximate to 34 kDa and that the mature form released by Meth A cells corresponds to approximate to 20 kDa. Purified recombinant mature EMAP II (EMAP II, approximate to 20 kDa form) activated endothelial cells with resulting elevation of cytosolic free calcium concentration, release of von Willebrand factor, induction of tissue factor, and expression of the adhesion molecules E-selectin and P-selectin. Neutrophils exposed to EMAP II demonstrated elevated cytosolic free calcium concentration, peroxidase generation, andchemotaxis. EMAP II also activated mononuclear phagocytes elevating cytosolic free calcium concentration, inducing tumor necrosis factor-alpha (TNF) and tissue factor, and stimulating chemotaxis. Systemic infusion of EMAP II into C3H/HeJ or Balb/c mice was associated with systemic toxicity, pulmonary congestion, and the appearance of TNF, interleukin-1 and -6 in the plasma. A single intratumor injection of EMAP II into Meth A sarcomas induced acute thrombohemorrhage and partial tumor regression. Local injection of EMAP II into a tumor resistant to the effects of TNF murine mammary carcinoma, rendered it sensitive to subsequently administered TNF, which resulted in acute thrombohemorrhage and partial regression. These data suggest that recombinant EMAP II, a tumor-derived cytokine, has properties of a proinflammatory mediator with the capacity to prime the tumor vasculature for a locally destructive process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:55:01