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Titolo:
EXPRESSION OF HUMAN APOLIPOPROTEIN B90 IN TRANSGENIC MICE - DEMONSTRATION THAT APOLIPOPROTEIN B90 LACES THE STRUCTURAL REQUIREMENTS TO FORMLIPOPROTEIN(A)
Autore:
MCCORMICK SPA; LINTON MF; HOBBS HH; TAYLOR S; CURTISS LK; YOUNG SG;
Indirizzi:
UNIV CALIF SAN FRANCISCO,DEPT MED,GLADSTONE INST CARDIOVASC DIS,POB 419100 SAN FRANCISCO CA 94141 UNIV CALIF SAN FRANCISCO,DEPT MED,GLADSTONE INST CARDIOVASC DIS SAN FRANCISCO CA 94141 UNIV CALIF SAN FRANCISCO,DEPT MED,INST CARDIOVASC RES SAN FRANCISCO CA 94141 SCRIPPS CLIN & RES INST,DEPT IMMUNOL LA JOLLA CA 92037 UNIV TEXAS,SW MED CTR DALLAS TX 75235 SCRIPPS CLIN & RES INST,DEPT VASC BIOL IMM17 LA JOLLA CA 92037
Titolo Testata:
The Journal of biological chemistry
fascicolo: 39, volume: 269, anno: 1994,
pagine: 24284 - 24289
SICI:
0021-9258(1994)269:39<24284:EOHABI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; FAMILIAL HYPOBETALIPOPROTEINEMIA; MYOCARDIAL-INFARCTION; HEART-DISEASE; B GENE; BINDING; PLASMA; ANTIBODIES; CYSTEINE; RISK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
S.P.A. Mccormick et al., "EXPRESSION OF HUMAN APOLIPOPROTEIN B90 IN TRANSGENIC MICE - DEMONSTRATION THAT APOLIPOPROTEIN B90 LACES THE STRUCTURAL REQUIREMENTS TO FORMLIPOPROTEIN(A)", The Journal of biological chemistry, 269(39), 1994, pp. 24284-24289

Abstract

Lipoprotein(a) (Lp(a)) is a lipoprotein formed by the disulfide linkage of apolipoprotein(a) (apo(a)) to the apoB100 of a low density lipoprotein particle. Earlier site-directed mutagenesis studies of apo(a) demonstrated that apo(a) cysteine 4057 is required for the disulfide linkage; however, the cysteine residue within apoB100 that is involved in the disulfide bond has not been identified. We previously demonstrated that the apoB100 produced by human apoB transgenic mice binds to apo(a) and forms Lp(a) (Linton, M. F., Farese, R. V., Jr., Chiesa, G., Grass, D. S., Chin, P., Hammer, R. E., Hobbs, H. H., and Young, S. G. (1993) J. Clin. Invest. 99, 3029-3037). To further explore the structural features of human apoB that are required for the formation of Lp(a), we used a transposon-interrupted human apoB gene clone to develop transgenic mice that express high levels of a truncated form of human apoB, apoB90, which contains the amino-terminal 4084 amino acids of apoB. In vitro incubation of apo(a) with the plasma of human apoB90 transgenic mice did not yield Lp(a), as judged by Western blots of SDS-polyacrylamide gels or by a monoclonal antibody-based radioimmunoassay. In contrast, incubation of apo(a) with the plasma of a mouse that expressed an equivalent amount of the full-length apoB100 did yield Lp(a). Inaddition to these in vitro incubation studies, no Lp(a) could be detected in the plasma of a ''double transgenic'' mouse expressing both human apoB90 and apo(a). These data indicate that the carboxyl-terminal 10% of apoB100 contains amino acid sequences that are essential for the formation of Lp(a).

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Documento generato il 04/12/20 alle ore 05:42:11