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Titolo:
RECOGNITION OF MURINE INTEGRIN BETA(1) BY A RAT ANTI-STROMAL CELL MONOCLONAL-ANTIBODY
Autore:
WU XY; MIYAKE K; MEDINA KL; KINCADE PW; GIMBLE JM;
Indirizzi:
OKLAHOMA MED RES FDN,IMMUNOBIOL & CANC PROGRAM,825 NE 13TH ST OKLAHOMA CITY OK 73104 OKLAHOMA MED RES FDN,IMMUNOBIOL & CANC PROGRAM OKLAHOMA CITY OK 73104 SAGA MED SCH,DEPT IMMUNOL SAGA 849 JAPAN
Titolo Testata:
Hybridoma
fascicolo: 5, volume: 13, anno: 1994,
pagine: 409 - 416
SICI:
0272-457X(1994)13:5<409:ROMIBB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW; LYMPHO-HEMATOPOIESIS; FIBRONECTIN RECEPTOR; EXPRESSION; ADHESION; VLA-4; GLYCOSYLATION; ACTIVATION; PROTEINS; EPITOPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
X.Y. Wu et al., "RECOGNITION OF MURINE INTEGRIN BETA(1) BY A RAT ANTI-STROMAL CELL MONOCLONAL-ANTIBODY", Hybridoma, 13(5), 1994, pp. 409-416

Abstract

Previous studies with the rat monoclonal antibody KMI6 had localized its antigen in vivo to a discrete subpopulation of marrow stromal cells. The KMI6 antigen has now been identified as the murine homolog of integrin beta(1) by amino acid sequence analysis and by cross-reactivity with antiserum to the avian integrin beta(1). The relative tissue abundance of murine integrin beta(1) was determined by Western blot. Although immunoperoxidase staining of fixed murine hematopoietic tissues demonstrated an abundance of intracellular beta(1), few primary-derived cells of lymphohematopoietic origin were surface positive as assessed by immunofluorescence and flow cytometry. Fetal erythroblasts provided the only exception. In contrast, the antigen was readily detected on the surface of several cultured cell lines in association with a variety of or chains. The biochemical properties of the surface labeled murine integrin beta(1) were similar to those of its human counterpart,exhibiting an altered electrophoretic migration under reduced conditions or following N-glycanase treatment. The antibody recognition of the protein was insensitive to glycosylation state, presence of divalentcations, detergents, or transfer to a nitrocellulose membrane. However, on Western blot, the epitope was lost on reduction of the protein, suggesting that it is conformation dependent. These data indicate thatalthough KMI6 epitope is widely distributed, its surface expression in vivo may be restricted within lymphohemopoietic tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:50:49