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Titolo:
SARCOPLASMIC-RETICULUM CA-RELEASE CHANNEL AND ATP-SYNTHESIS ACTIVITIES ARE EARLY MYOCARDIAL MARKERS OF HEART-FAILURE PRODUCED BY RAPID VENTRICULAR PACING IN DOGS
Autore:
OBRIEN PJ; MOE GW; NOWACK LM; GRIMA EA; ARMSTRONG PW;
Indirizzi:
UNIV GUELPH,ONTARIO VET COLL,DEPT PATHOL GUELPH N1G 2W1 ON CANADA UNIV TORONTO,ST MICHAELS HOSP,DEPT MED,DIV CARDIOL TORONTO M5B 1W8 ONCANADA
Titolo Testata:
Canadian journal of physiology and pharmacology
fascicolo: 9, volume: 72, anno: 1994,
pagine: 999 - 1006
SICI:
0008-4212(1994)72:9<999:SCCAAA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TACHYCARDIA-INDUCED CARDIOMYOPATHY; CALCIUM CYCLING DEFECT; CREATINE-KINASE SYSTEM; DILATED CARDIOMYOPATHY; GENE-EXPRESSION; OVERLOAD; MODEL; SPECTROFLUOROMETRY; SEQUESTRATION; RECOVERY;
Keywords:
CA-RELEASE CHANNEL; SARCOPLASMIC RETICULUM; RAPID VENTRICULAR PACING; CONGESTIVE HEART FAILURE; CA ATPASE PUMP; MITOCHONDRIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Obrien et al., "SARCOPLASMIC-RETICULUM CA-RELEASE CHANNEL AND ATP-SYNTHESIS ACTIVITIES ARE EARLY MYOCARDIAL MARKERS OF HEART-FAILURE PRODUCED BY RAPID VENTRICULAR PACING IN DOGS", Canadian journal of physiology and pharmacology, 72(9), 1994, pp. 999-1006

Abstract

The contraction-relaxation cycle of the heart is dependent on a cycleof ATP production and utilization and a cycle of Ca uptake and Ca release by the sarcoplasmic reticulum (SR). Heart failure (HF) is associated with abnormalities of myocardial Ca and ATP cycling, but the time course of their development is unknown. This study tested the hypothesis that, compared with ATP-utilizing and Ca-uptake activities, decreases in ATP-synthesis and Ca-release activities occurred earlier in the development of HF and persisted longer during recovery from HF. HF wasinduced by right ventricular pacing of dogs at 250 beats/min. Dogs were studied after 1 week of pacing (n = 8, early HF), at HF (n = 11, severe HF), and 4 weeks after cessation of pacing (n = 9) and were compared with dogs not subjected to pacing. At early HF, there were decreased activities (p < 0.05) of the SR Ca-release channel (rate constant from 199 +/- 36 x 10(-4) to 90 +/- 16 x 10(-4) s(-1)), mitochondrial ATP synthesis (from 11.2 +/- 2.4 to 7.0 +/- 2.2 international units (IU)/g), and creatine kinase (CK) from 2028 +/- 266 to 1811 +/- 79 IU/g). The decreased Ca-channel activity was due to a 32% decrease in maximalactivity (rate constant from 249 +/- 50 x 10(-4) to 170 +/- 29 X 10(-4) s(-1)) and to a 2-fold increase (from 19.1 +/- 12.4 to 42.0 +/- 14.2%) in inhibition of maximal channel activity (p < 0.05). At severe HF, Ca-uptake (rate constant from 407 +/- 41 x 10(-4) to 296 +/- 77 x 10(-4) s(-1)) and ATP-utilization activities also became depressed (from27.2 +/- 3.3 to 20.3 +/- 1.9 IU/g), and CK further decreased to 1321 /- 241 IU/g (p < 0.05). Four weeks after cessation of pacing, only total Ca-cycling (sum of Ca uptake and Ca release), Ca-uptake, and CK activities were significantly recovered (p < 0.05). Left ventricular ejection fraction was significantly correlated with total Ca cycling (n =12, r = 0.68, p < 0.02), Ca-channel inhibition (n = 12, r = -0.60, p < 0.04), and basal ATPase (n = 11, r = 0.69, p < 0.02). We conclude that biochemical measurements of ATP- and Ca-cycling activities correlate with myocardial performance, and that compared with ATP-utilization and Ca-uptake activities, inhibition of ATP synthesis and Ca release occurs earlier in the development of HF and persists longer during recovery from HF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:48:13