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Titolo:
LEADING VERSUS LAGGING-STRAND MUTAGENESIS INDUCED BY 7,8-DIHYDRO-8-OXO-2'-DEOXYGUANOSINE IN ESCHERICHIA-COLI
Autore:
WAGNER J; KAMIYA H; FUCHS RPP;
Indirizzi:
HOP UNIV,CANC & MUTAGENESE MOL & STRUCT UNITE PROPRE RECH,CNRS,ICAD,BP 426 F-67091 STRASBOURG FRANCE HOP UNIV,CANC & MUTAGENESE MOL & STRUCT UNITE PROPRE RECH,CNRS,ICAD F-67091 STRASBOURG FRANCE UNIV OCCUPAT & ENVIRONM HLTH,DEPT ENVIRONM ONCOL,INST IND ECOL SCI,YAHATANISHI KU KITAKYUSHU FUKUOKA 807 JAPAN
Titolo Testata:
Journal of Molecular Biology
fascicolo: 3, volume: 265, anno: 1997,
pagine: 302 - 309
SICI:
0022-2836(1997)265:3<302:LVLMIB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLUE PLUS LIGHT; SINGLE ACETYLAMINOFLUORENE ADDUCT; MUTATION HOT SPOT; C-HA-RAS; IONIZING-RADIATION; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; DNA-REPLICATION; VISIBLE-LIGHT; FPG PROTEIN; GENE;
Keywords:
8-OXODG; REPAIR; MUTATION FREQUENCY; REPLICATION ASYMMETRY; QUANTITATIVE PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
J. Wagner et al., "LEADING VERSUS LAGGING-STRAND MUTAGENESIS INDUCED BY 7,8-DIHYDRO-8-OXO-2'-DEOXYGUANOSINE IN ESCHERICHIA-COLI", Journal of Molecular Biology, 265(3), 1997, pp. 302-309

Abstract

We have previously shown that a single N-2-acetylaminofluorene (AAF) adduct bound to the C-8 position of a guanine residue located within plasmids containing the unidirectional ColE1 origin of replication induces a 20-fold higher mutation frequency when the adduct is located in the lagging strand as compared to the leading strand. In this study, single 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxodG) lesions have been introduced in the leading and lagging strand orientation within the same sequence context as for the AAF adducts. The induced frequency of guanine to thymine transversions has been measured, using a specific PCR-based quantitative assay, in strains deficient in the repair of the oxidative lesion. The potential involvement of the UvrABC excision repair system in the removal of 8-oxodG has also been investigated and ruled out. Concerning the mutation frequency asymmetry, in contrast to AAF adducts, 8-oxodG adducts induce the same mutation frequency, irrespective of their location in the leading or lagging strands. This striking difference between 8-oxodG and dGuo-C8-AAF adducts is discussed interms of their differential capacity to block DNA replication. (C) 1997 Academic Press Limited

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:46:23