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Titolo:
INDUCED HYPOXIA IN RAT PULP AND PERIAPEX DEMONSTRATED BY H-3 MISONIDAZOLE RETENTION
Autore:
BAUMGARDNER KR; WALTON RE; OSBORNE JW; BORN JL;
Indirizzi:
UNIV MICHIGAN,SCH DENT,DEPT CARIOL RESTORAT SCI & ENDODONT ANN ARBOR MI 48109 UNIV IOWA,COLL DENT,DEPT ENDODONT IOWA CITY IA 52242 UNIV IOWA,RADIAT RES LAB,MED LAB 14 IOWA CITY IA 52242 UNIV NEW MEXICO,COLL PHARM ALBUQUERQUE NM 87131
Titolo Testata:
Journal of dental research
fascicolo: 10, volume: 75, anno: 1996,
pagine: 1753 - 1760
SICI:
0022-0345(1996)75:10<1753:IHIRPA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE-TISSUES; MISONIDAZOLE BINDING; BIOREDUCTIVE DRUGS; POSSIBLE MECHANISM; HYPOBARIC HYPOXIA; H-3 MISONIDAZOLE; TUMOR HYPOXIA; CYTO-TOXICITY; SIDE-CHAIN; CELLS;
Keywords:
HYPOXIA; MISONIDAZOLE; DENTAL PULP; ODONTOBLASTS; AMELOBLASTS; MALE SPRAGUE-DAWLEY RATS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
K.R. Baumgardner et al., "INDUCED HYPOXIA IN RAT PULP AND PERIAPEX DEMONSTRATED BY H-3 MISONIDAZOLE RETENTION", Journal of dental research, 75(10), 1996, pp. 1753-1760

Abstract

Cellular hypoxia may be a useful indication of tissue distress in thedental pulp that could be used to investigate the early stages of pulpal responses. Tritiated misonidazole (H-3-MISO) is a marker which preferentially labels cells with decreased oxygen tension (hypoxia). The experiments reported here were carried out to determine whether this agent could distinguish between hypoxic and normoxic pulp and periapical tissues. Rats were injected intra-peritoneally with either H-3-MISO,unlabeled MISO, or saline, then divided into normoxic, hypoxic, and control groups. Normoxic animals were maintained at ambient pressure. We induced hypoxia by maintaining animals in a hypobaric chamber at 0.5atm for 24 hrs. H-3-MISO retention was assessed by quantitative analysis of tissue autoradiographs. H-3-MISO retention rates in normoxic animals showed little variation except for increased retention in matureameloblasts and immature odontoblasts in the continually erupting incisor. In both incisor and molar pulps, hypobaric hypoxia significantlyincreased H-3-MTSO retention when compared with normoxic controls. Hypobaric hypoxia also resulted in intense H-3-MISO retention in cellular cementum, periodontal ligament, osteocytes, and, occasionally, in molar pulp horn odontoblasts. This study demonstrated that, with standard autoradiographic techniques, H-3-MISO can label induced hypoxic disturbances in the pulp and surrounding periodontium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:01:51