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Titolo:
EXTRAMEDULLARY PLASMACYTOMA OF THE HEAD AND NECK - USE OF PARAFFIN SECTIONS TO ASSESS CLONALITY WITH IN-SITU HYBRIDIZATION, GROWTH FRACTION, AND THE PRESENCE OF EPSTEIN-BARR-VIRUS
Autore:
AGUILERA NS; KAPADIA SB; NALESNIK MA; SWERDLOW SH;
Indirizzi:
UNIV PITTSBURGH,PRESBYTERIAN HOSP,MED CTR,DEPT PATHOL,200 LOTHROP ST PITTSBURGH PA 15213 UNIV PITTSBURGH,SCH MED,DEPT PATHOL,DIV HEMATOPATHOL PITTSBURGH PA 00000 UNIV PITTSBURGH,SCH MED,DEPT PATHOL,DIV ANAT PATHOL PITTSBURGH PA 00000 UNIV PITTSBURGH,SCH MED,DEPT PATHOL,DIV TRANSPLANT PATHOL PITTSBURGH PA 00000
Titolo Testata:
Modern pathology
fascicolo: 5, volume: 8, anno: 1995,
pagine: 503 - 508
SICI:
0893-3952(1995)8:5<503:EPOTHA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL NUCLEAR ANTIGEN; CHAIN MESSENGER-RNA; POSTTRANSPLANT LYMPHOPROLIFERATIVE DISORDERS; EXTRA-MEDULLARY PLASMACYTOMA; NON-HODGKINS-LYMPHOMA; INSITU HYBRIDIZATION; MULTIPLE-MYELOMA; IMMUNOBLASTIC LYMPHOMA; INFECTED PATIENTS; EMBEDDED TISSUES;
Keywords:
EXTRAMEDULLARY PLASMACYTOMA; IN SITU HYBRIDIZATION; IMMUNOHISTOCHEMISTRY; EBV; PROLIFERATING CELL NUCLEAR ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
N.S. Aguilera et al., "EXTRAMEDULLARY PLASMACYTOMA OF THE HEAD AND NECK - USE OF PARAFFIN SECTIONS TO ASSESS CLONALITY WITH IN-SITU HYBRIDIZATION, GROWTH FRACTION, AND THE PRESENCE OF EPSTEIN-BARR-VIRUS", Modern pathology, 8(5), 1995, pp. 503-508

Abstract

The diagnosis of extramedullary plasmacytomas (EMP) is usually easy to confirm with immunohistochemical stains for kappa and lambda. In some cases, however, immunostains are problematic. In addition, prognostic features are not well described nor is it known whether EMP are associated with the Epstein-Barr virus. Therefore, 23 EMP of the head and neck (from 20 patients) were studied to (1) compare a non-isotopic paraffin section in situ hybridization technique for kappa and lambda mRNA with standard immunohistochemical techniques for assessing light chain expression, (2) compare the histologic grade to the proliferative fraction using an antibody for the proliferating cell nuclear antigen, and (3) determine the frequency of Epstein-Barr virus (EBV) association using probes for both EBV DNA (EBV NOT 1) and RNA (EBER-1). Light chain class restriction was demonstrated in 22/23 biopsies by in situ hybridization and in 21/23 biopsies by standard immunohistochemical techniques. Five of the six biopsies of well-differentiated EMP had proliferating cell nuclear antigen scores of 0 to 10% positive cells and onehad 11 to 25% positive cells compared with greater than 75% in the one poorly differentiated EMP. Of 15 moderately differentiated EMP, 10 had proliferating cell nuclear antigen scores of 11 to 75%, and 5 had scores of greater than 75%. EBV DNA was detected in 1/23 biopsies and EBV RNA in 4/23 biopsies (3 patients). Thus, non-isotopic in situ hybridization is a useful technique to document clonality of plasma cells in routinely fixed, paraffin-embedded sections. Unlike routine immunohistochemistry, in situ hybridization avoids the problem of detecting stromal or nonspecific uptake of immunoglobulin. Use of proliferating cell nuclear antigen to study the proliferative rate of EMP revealed a wide spectrum of results that appeared to correlate with histologic grade and could potentially be of prognostic importance. EBV does not appear to be of major importance in understanding the pathophysiology of EMP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 13:37:19