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Titolo:
SIMULTANEOUS QUANTITATION OF AMPHETAMINE AND 4'-HYDROXYAMPHETAMINE BYHIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Autore:
LAW MYL; MOODY DE;
Indirizzi:
BIOMED POLYMER BLDG,ROOM 490 SALT LAKE CITY UT 84112 UNIV UTAH,DEPT PHARMACOL & TOXICOL,CTR HUMAN TOXICOL SALT LAKE CITY UT 84112
Titolo Testata:
Journal of liquid chromatography
fascicolo: 10, volume: 18, anno: 1995,
pagine: 2029 - 2043
SICI:
0148-3919(1995)18:10<2029:SQOAA4>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
AQUEOUS IONIC ELUENTS; BASIC DRUGS; RAT URINE; METABOLISM; METHAMPHETAMINE; RETENTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M.Y.L. Law e D.E. Moody, "SIMULTANEOUS QUANTITATION OF AMPHETAMINE AND 4'-HYDROXYAMPHETAMINE BYHIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY", Journal of liquid chromatography, 18(10), 1995, pp. 2029-2043

Abstract

A relatively simple HPLC procedure used to detect and quantify amphetamine and 4'-hydroxyamphetamine in rat urine has been developed. Following Bond Elut(R) solid phase extraction, the two analytes - amphetamine and 4'-hydroxyamphetamine, and the two internal standards - methamphetamine and 4'-hydroxymethamphetamine were separated by HPLC using a phenyl column and detected by UV at 215 nm. The limit of quantitation for amphetamine and 4'-hydroxyamphetamine was 0.92 and 0.81 mu g/ml, respectively. Based on replicated analysis of controls at 1.6, 6.5, and16.0 mu g/ml, the method is accurate (94 - 103% of target), and precise (% CVs of 1.3 - 5.2). The linear range of the assay is suitable forthe quantitation of both analytes at urine concentrations that commonly result from administration of a single dose of amphetamine to the rat.

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Documento generato il 05/07/20 alle ore 07:05:13