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Titolo:
EFFECTS OF AEROSOLIZED AMILORIDE ON MUCOCILIARY TRANSPORT VELOCITY AND TRANSEPITHELIAL POTENTIAL DIFFERENCE IN ISOLATED FROG PALATE
Autore:
FESTA E; MACCHIONE M; PAIVA PSO; SALDIVA PHN; KING M;
Indirizzi:
UNIV ALBERTA,DEPT MED,PULM RES GRP,173 HERITAGE MED RES CTR EDMONTON AB T6G 2S2 CANADA
Titolo Testata:
Journal of aerosol medicine
fascicolo: 2, volume: 8, anno: 1995,
pagine: 167 - 176
SICI:
0894-2684(1995)8:2<167:EOAAOM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Keywords:
MUCOCILIARY CLEARANCE; TRANSEPITHELIAL POTENTIAL DIFFERENCE; ION TRANSPORT; FROG PALATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
E. Festa et al., "EFFECTS OF AEROSOLIZED AMILORIDE ON MUCOCILIARY TRANSPORT VELOCITY AND TRANSEPITHELIAL POTENTIAL DIFFERENCE IN ISOLATED FROG PALATE", Journal of aerosol medicine, 8(2), 1995, pp. 167-176

Abstract

An in vitro dose-response study was performed to determine the effects of aerosolized amiloride on transepithelial potential difference andmucociliary velocity. A preparation of isolated frog palate (Rana sculenta) was used for two groups: one submitted to amiloride nebulization (n = 7) and a control group submitted to vehicle (NaCl 0.45%) nebulization (n = 7). Transepithelial potential difference was measured by means of agar-filled microelectrodes connected to the high input of a grounded electrometer. Mucociliary velocity was determined by monitoring the movement of aluminum disks as tracer particles. Amiloride nebulization caused a significant dose-dependent increase in mucociliary velocity when compared to the control group (p < 0.05). The amiloride also turned the luminal surface more positive, promoting a decrease (in absolute value) in transepithelial potential difference when compared to the control group (p < 0.05). A positive and continuous (quadratic) association between mucociliary velocity and transepithelial potentialdifference was verified in the group treated with amiloride (p = 0.019). The results presented are in agreement with a decrease in mucus rigidity due to increased hydration of the mucous gel, induced by the blockade of sodium channels by the drug. In the range of concentrations used in this study (10(-7) to 10(-3) M amiloride in NaCl 0.45% solution) there was no evidence of uncoupling between the mucous gel and the tips of the cilia. The model should prove to be valuable for studying agents which can act as modulators of transepithelial ionic conductance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:40:06