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Titolo:
POLYNUCLEOTIDES COMPENSATE FOR IMPAIRED T-DEPENDENT ANTIBODY-PRODUCTION INDUCED IN C57BL 6 MICE BY A NUCLEOTIDE-FREE DIET BOTH IN-VIVO AND IN-VITRO, BUT A MONONUCLEOTIDE-NUCLEOSIDE MIXTURE IS EFFECTIVE ONLY IN-VIVO/
Autore:
JYONOUCHI H; SUN SN; ZHANGSHANBHAG L; YOKOYAMA H;
Indirizzi:
UNIV MINNESOTA,DEPT PEDIAT,DIV IMMUNOL MINNEAPOLIS MN 55455 OTSUKA PHARMACEUT FACTORY INC,NUTR RES LAB TOKUSHIMA 772 JAPAN
Titolo Testata:
The Journal of nutrition
fascicolo: 6, volume: 125, anno: 1995,
pagine: 1578 - 1586
SICI:
0022-3166(1995)125:6<1578:PCFITA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTES;
Keywords:
NUCLEOTIDES; CYTOKINE; MICE; T HELPER CELLS; T-DEPENDENT ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
H. Jyonouchi et al., "POLYNUCLEOTIDES COMPENSATE FOR IMPAIRED T-DEPENDENT ANTIBODY-PRODUCTION INDUCED IN C57BL 6 MICE BY A NUCLEOTIDE-FREE DIET BOTH IN-VIVO AND IN-VITRO, BUT A MONONUCLEOTIDE-NUCLEOSIDE MIXTURE IS EFFECTIVE ONLY IN-VIVO/", The Journal of nutrition, 125(6), 1995, pp. 1578-1586

Abstract

Actions of nucleotides on in vitro humoral immune responses were studied in mice fed a nucleotide-free diet, a nucleotide-free diet plus a mononucleotide-nucleoside mixture or a nucleotide-free diet plus yeastRNA (polynucleotides). Cultured spleen cells from mice fed a nucleotide-free diet produced fewer numbers of antibody-secreting cells in response to a T-dependent antigen, compared with those from controls fed nucleotide-supplemented diets. Immunoglobulin M concentrations in these supernatants were significantly lower in cultured cells from mice fed the nucleotide-free diet or the nucleotide-free diet plus the mononucleotide/ nucleoside mixture compared with concentrations in cells from mice fed the nucleotide-free diet plus RNA. Concanavalin A-potentiated cytokine (interleukin-4 and interleukin-5) production by purified Thelper cells was also lower in cultured cells from mice fed a nucleotide-free diet than in those from mice fed nucleotide-supplemented diets. In vivo supplementation with the mononucleotide/nucleoside mixture restored impaired in vitro antibody and concanavalin A-potentiated cytokine production in mice fed a nucleotide-free diet. However, additionof RNA to the culture enhanced antibody production in spleen cells from mice of all diet groups. Supplementing the culture with RNA did notenhance mitogen-potentiated cytokine production. This in vitro actionof RNA was retained after the removal of oligonucleotides (molecular weight <1000), but was reduced by modification of bases and cleavage of phosphodiester bonds of RNA. Thus the in vitro action of RNA is mainly attributed to polynucleotides, indicating their potential role in modulation of local humoral immune responses in the body.

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Documento generato il 04/12/20 alle ore 09:50:27