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Titolo:
DISTRIBUTION AND ACTIVITY OF CALCINEURIN IN RAT-TISSUES - EVIDENCE FOR POSTTRANSCRIPTIONAL REGULATION OF TESTIS-SPECIFIC CALCINEURIN-B
Autore:
SU QX; ZHAO M; WEBER E; EUGSTER HP; RYFFEL B;
Indirizzi:
UNIV ZURICH,INST TOXICOL,SCHORENSTR 16 CH-8603 SCHWERZENBACH SWITZERLAND UNIV ZURICH,INST TOXICOL CH-8603 SCHWERZENBACH SWITZERLAND INST PATHOL BERN SWITZERLAND ETH ZURICH SCHWERZENBACH SWITZERLAND
Titolo Testata:
European journal of biochemistry
fascicolo: 2, volume: 230, anno: 1995,
pagine: 469 - 474
SICI:
0014-2956(1995)230:2<469:DAAOCI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT PROTEIN-KINASE; CYCLOSPORINE-A; BRAIN; IDENTIFICATION; PHOSPHATASE; PHOSPHORYLATION; LOCALIZATION; ACTIVATION; EXPRESSION; DOMAIN;
Keywords:
CALCINEURIN; CYCLOSPORINE; TACROLIMUS; RAPAMYCIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
Q.X. Su et al., "DISTRIBUTION AND ACTIVITY OF CALCINEURIN IN RAT-TISSUES - EVIDENCE FOR POSTTRANSCRIPTIONAL REGULATION OF TESTIS-SPECIFIC CALCINEURIN-B", European journal of biochemistry, 230(2), 1995, pp. 469-474

Abstract

Calcineurin (CN), a Ca2+/calmodulin-regulated phophatase 2B, plays animportant role in many biological processes including T-cell signal transduction. In the present study, the distribution and activity of CNwere investigated in rat tissues. CN has a wide tissue distribution, as measured by enzyme-linked immunosorbent assay. CN concentrations are 0.2-0.6 mu g/mg protein in most tissues, while the brain contains 3-10-fold higher concentrations. Immunohistochemical analyses using a monoclonal antibody to CN B subunit reveals that CN is not evenly distributed but concentrated in specific cells, especially in the brain, kidneys and testis. The specific enzymic activity of CN in tissues is around 10 pmol . min . mg protein(-1), except in brain and liver (60 pmol. min(-1) . mg protein(-1) compared to 3.6 pmol . min(-1) . mg protein(-1)). The immunosuppressants cyclosporin A and tacrolimus, but not rapamycin, inhibit the phosphatase activity of CN derived from most tissues tested, while CN activity from liver was resistant to cyclosporinA. Furthermore, transcripts and protein of the common CN B subunit and of the testis-specific form of CN B subunit were analyzed. The common CN B subunit transcripts and protein are detected in all tissues. Transcripts for the 'testis-specific' CN B subunit are also found in brain, lung, thymus and heart, while the protein is only detected in testis. This indicates that the testis-specific CN B subunit gene expression is regulated at both transcriptional and posttranscriptional levels. The findings demonstrate that CN is a widely distributed protein phosphatase and that its activity is regulated in a tissue-specific manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 02:10:39