Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MUTATIONS AT THE C-TERMINAL ISOLEUCINE AND OTHER POTENTIAL IRON LIGANDS OF 5-LIPOXYGENASE
Autore:
HAMMARBERG T; ZHANG YY; LIND B; RADMARK O; SAMUELSSON B;
Indirizzi:
KAROLINSKA INST,DEPT MED BIOCHEM & BIOPHYS S-17177 STOCKHOLM SWEDEN KAROLINSKA INST,INST ENVIRONM MED S-10401 STOCKHOLM SWEDEN
Titolo Testata:
European journal of biochemistry
fascicolo: 2, volume: 230, anno: 1995,
pagine: 401 - 407
SICI:
0014-2956(1995)230:2<401:MATCIA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; ACTIVE-SITE; SOYBEAN LIPOXYGENASE-1; MUTAGENESIS; RESIDUES; 12-LIPOXYGENASE; EXPRESSION; LEUKOTRIENES; PROTEINS; BINDING;
Keywords:
LIPOXYGENASE; ARACHDONIC ACID; EICOSANOID; MUTAGENESIS; NONHEME IRON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
T. Hammarberg et al., "MUTATIONS AT THE C-TERMINAL ISOLEUCINE AND OTHER POTENTIAL IRON LIGANDS OF 5-LIPOXYGENASE", European journal of biochemistry, 230(2), 1995, pp. 401-407

Abstract

The non-heme iron centre in human 5-lipoxygenase was studied. Recombinant enzyme was expressed in Escherichia coli, purified and assayed for iron content and enzyme activity. For non-mutated enzyme, the iron content was 1.01 +/- 0.19 mol/mol. Deletion of the C-terminal Ile673 resulted in an iron content of 0.03 +/- 0.07 mol/mol and undetectable lipoxygenase activity. Mutations at His367, Glu376 and Asn554 led to drastically decreased enzyme activity (<2% of non-mutated control) but iron was still present. In addition to Glu376, eight other conserved acidic residues (Asp/Glu) in 5-lipoxygenase were replaced, none of which was crucial for enzyme activity. We conclude that Ile673 is an iron Ligand in 5-lipoxygenase, while our results do not support that Glu376 or Asn554 have this function. The possible role of His367 as a replaceable iron ligand is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:56:11