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Titolo:
CILIARY BEAT FREQUENCY OF HAMSTER OVIDUCTS IS DECREASED IN-VITRO BY EXPOSURE TO SOLUTIONS OF MAINSTREAM AND SIDESTREAM CIGARETTE-SMOKE
Autore:
KNOLL M; SHAOULIAN R; MAGERS T; TALBOT P;
Indirizzi:
UNIV CALIF RIVERSIDE,DEPT BIOL RIVERSIDE CA 92521 UNIV CALIF RIVERSIDE,DEPT BIOL RIVERSIDE CA 92521
Titolo Testata:
Biology of reproduction
fascicolo: 1, volume: 53, anno: 1995,
pagine: 29 - 37
SICI:
0006-3363(1995)53:1<29:CBFOHO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUCOCILIARY CLEARANCE; ECTOPIC PREGNANCY; TUBAL PREGNANCY; NICOTINE; COTININE; INVITRO; FLUID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
M. Knoll et al., "CILIARY BEAT FREQUENCY OF HAMSTER OVIDUCTS IS DECREASED IN-VITRO BY EXPOSURE TO SOLUTIONS OF MAINSTREAM AND SIDESTREAM CIGARETTE-SMOKE", Biology of reproduction, 53(1), 1995, pp. 29-37

Abstract

Epidemiological data support a correlation between smoking and increased incidence of ectopic pregnancy, yet the causal mechanism responsible for this relationship is unknown. The purpose of this study was to examine the effect of solutions containing dissolved mainstream (MS) or sidestream (SS) cigarette smoke on ciliary beat frequencies (CBF) inexplants of hamster oviducts. MS smoke is the puff inhaled by an active smoker, while SS smoke leaves the burning end of cigarette. SS smoke is inhaled by both active and passive smokers. Experiments were performed in handmade perfusion chambers using infundibula from hamster oviducts. After a short incubation in Earle's balanced salt solution containing HEPES buffer (EBSS-H), chambers were flushed with one of six types of smoke solution prepared in EBSS-H, and incubation continued 19min. A second perfusion (washout) was then done using EBSS-H alone todetermine whether effects induced by the smoke solutions could be reversed. CBF were determined at three times in both the smoke and washout solutions, and means were compared to values obtained in the initialEBSS-H incubation. All smoke solutions except the SS particulate solution inhibited CBF in a dose-dependent manner. Whole MS and whole SS smoke solution at the highest strength tested caused the greatest inhibition and in some cases completely stopped ciliary beating. Both single-strength and 0.1-strength MS gas phase solutions, which contained concentrations of nicotine in the range found in typical human smokers, produced about 50% inhibition of ciliary beating. Inhibition was generally seen within 2-12 min of adding smoke solutions. In all cases, washout of smoke solution caused an increase in CBF. In some washouts, CBF returned to initial values found in EBSS-H within 2 min of incubation in the recovery medium. However, in both MS and SS whole smoke solutions, CBF did not return to the initial EBSS-H values during the washout interval. In SS gas phase solutions, inhibition was not observed during exposure to the smoke solution but did occur during washout, suggesting that the mechanism of action of SS gas phase solutions differs from the other types of smoke solution tested. These results show thatcomponents in both MS and SS cigarette smoke can inhibit beating of oviductal cilia in vitro and that inhibition can be at least partially reversed when the smoke solution is replaced by EBSS-H.

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Documento generato il 26/11/20 alle ore 20:26:58