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Titolo:
PRESENCE OF NEGATIVE TORSIONAL TENSION IN THE PROMOTER REGION OF THE TRANSCRIPTIONALLY POISED DIHYDROFOLATE-REDUCTASE GENE IN-VIVO
Autore:
LJUNGMAN M; HANAWALT PC;
Indirizzi:
UNIV MICHIGAN,MED CTR,DEPT RADIAT ONCOL,DIV CANC BIOL,1331 E ANN ST ANN ARBOR MI 48109 STANFORD UNIV,DEPT BIOL SCI STANFORD CA 94305
Titolo Testata:
Nucleic acids research
fascicolo: 10, volume: 23, anno: 1995,
pagine: 1782 - 1789
SICI:
0305-1048(1995)23:10<1782:PONTTI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA POLYMERASE-II; 5,6-DICHLORO-1-BETA-D-RIBOFURANOSYLBENZIMIDAZOLE INHIBITS TRANSCRIPTION; HETEROGENEOUS NUCLEAR-RNA; HAMSTER OVARY CELLS; DNA STRAND BREAKS; HEAT-SHOCK GENES; MESSENGER-RNA; HELA-CELLS; INVITRO TRANSCRIPTION; HISTONE ACETYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
78
Recensione:
Indirizzi per estratti:
Citazione:
M. Ljungman e P.C. Hanawalt, "PRESENCE OF NEGATIVE TORSIONAL TENSION IN THE PROMOTER REGION OF THE TRANSCRIPTIONALLY POISED DIHYDROFOLATE-REDUCTASE GENE IN-VIVO", Nucleic acids research, 23(10), 1995, pp. 1782-1789

Abstract

DNA topology has been suggested to play an important role in the process of transcription. Negative torsional tension has been shown to stimulate both pre-initiation complex formation and promoter clearance onplasmid DNA in vitro. We recently showed that genomic DNA in human cells contains localized torsional tension. In the present study we havefurther characterized and mapped torsional tension in the dihydrofotate reductase (DHFR) gene in Chinese hamster ovary (CHO) cells and investigated the effects of differential rates of transcription on the magnitude and location of this tension. Using psoralen photo-cross-linking in conjunction with X-irradiation, we found that relaxable psoralen hypersensitivity was specifically localized to the promoter region of the serum-regulated DHFR gene in serum-stimulated, but not in serum-starved, cells. Moreover, this hypersensitivity did not appear to be caused by transcription elongation, since it persisted in cells in which transcription of the DHFR gene had been reduced by the transcription inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB). We suggest that the generation of negative torsional tension in DNA may play an important role in gene regulation by poising genes for transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:06:59