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Titolo:
SEQUENCING OF AN UPSTREAM REGION OF THE HUMAN HLA-DRA GENE CONTAININGX' AND Y' BOXES
Autore:
FERIOTTO G; MISCHIATI C; BIANCHI N; RUTIGLIANO C; GIACOMINI P; GAMBARI R;
Indirizzi:
UNIV FERRARA,DEPT BIOCHEM & MOLEC BIOL,VIA I BORSARI 46 I-44100 FERRARA ITALY UNIV FERRARA,DEPT BIOCHEM & MOLEC BIOL I-44100 FERRARA ITALY UNIV FERRARA,CTR INTERDIPARTIMENTAL BIOTECNOL I-44100 FERRARA ITALY IST REGINA ELENA,IMMUNOL LAB I-00161 ROME ITALY
Titolo Testata:
Nucleic acids research
fascicolo: 10, volume: 23, anno: 1995,
pagine: 1671 - 1678
SICI:
0305-1048(1995)23:10<1671:SOAURO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; CLASS-II GENE; DNA-BINDING PROTEINS; TISSUE-SPECIFIC EXPRESSION; RNA POLYMERASE-II; TRANSGENIC MICE; ALPHA GENE; REGULATORY REGIONS; HEAVY-CHAIN; B-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
G. Feriotto et al., "SEQUENCING OF AN UPSTREAM REGION OF THE HUMAN HLA-DRA GENE CONTAININGX' AND Y' BOXES", Nucleic acids research, 23(10), 1995, pp. 1671-1678

Abstract

In this paper we report the characterization of a newly sequenced 5' upstream region of the human HLA-DRA gene, We performed (i) search fortranscription factor motifs, (ii) analysis of CpG display and observed/expected frequency ratios, (iii) search for regions homologous to the 5' upstream sequences of the murine EA gene, (iv) DNase I footprinting experiments and (v) electrophoretic mobility shift assays. Our results demonstrate the existence, in the HLA-DRA gene, of Y' and X' boxeshighly homologous to the Y and X boxes present in MHC class II genes,but oriented in the opposite direction, These Y' and X' boxes have been conserved during the molecular evolution of both human HLA-DRA and murine EA genes. DNase I footprinting and gel retardation experiments suggest that the X' and Y' boxes of the HLA-DRA upstream gene region are specifically recognized by nuclear proteins that also bind to the Xand Y boxes of the HLA-DRA proximal promoter, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:31:20