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Titolo:
POLYAMINES MAY INCREASE THE PERCENTAGE OF IN-VITRO FERTILIZED MURINE OOCYTES THAT DEVELOP INTO BLASTOCYSTS
Autore:
MUZIKOVA E; CLARK DB;
Indirizzi:
MCMASTER UNIV,DEPT MED,ROOM 3V39,1200 MAIN ST W HAMILTON ON L8N 3Z5 CANADA MCMASTER UNIV,DEPT MED HAMILTON ON L8N 3Z5 CANADA MCMASTER UNIV,DEPT PATHOL HAMILTON ON L8N 3Z5 CANADA MCMASTER UNIV,DEPT OBSTET & GYNECOL HAMILTON ON L8N 3Z5 CANADA
Titolo Testata:
Human reproduction
fascicolo: 5, volume: 10, anno: 1995,
pagine: 1172 - 1177
SICI:
0268-1161(1995)10:5<1172:PMITPO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE PREIMPLANTATION EMBRYOS; DEVELOPMENT INVITRO; 2-CELL BLOCK; GROWTH-FACTORS; IMPLANTATION; SUPERNATANTS; THIOREDOXIN; METABOLISM; CLEAVAGE; SPERMINE;
Keywords:
BLASTOCYST; EMBRYO DEVELOPMENT; IN-VITRO FERTILIZATION; POLYAMINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
E. Muzikova e D.B. Clark, "POLYAMINES MAY INCREASE THE PERCENTAGE OF IN-VITRO FERTILIZED MURINE OOCYTES THAT DEVELOP INTO BLASTOCYSTS", Human reproduction, 10(5), 1995, pp. 1172-1177

Abstract

This study was designed to investigate whether the polyamines spermine, spermidine and putrescine can prevent the arrest of development of in-vitro fertilized murine oocytes from CD1 strain mice. Development in M16 or Brinster's medium with or without polyamine supplementation was assessed and the ability of blastocysts to attach and grow out on laminin was compared with in-vivo-generated embryo blastocysts. Oocytesfrom old (20 weeks) females showed lower rates of fertilization and subsequent development in comparison with oocytes from young (6-8 weeksold) females, but in both cases <5% of in-vitro fertilized oocytes achieved the blastocyst stage. In-vitro fertilized young oocytes showed enhanced development to the blastocyst stage with the addition of the polyamine putrescine, although lesser enhancement was seen with spermine and spermidine; this effect was only evident when the oocytes were grown in Brinster's medium, reflecting the need for a particular set of culture conditions for growth-enhancing agents to be effective. Blastocysts generated in putrescine-supplemented Brinster's medium were able to implant on laminin-coated surfaces in vitro and to respond to the cytokines colony-stimulating factor-1, epidermal growth factor and platelet-derived growth factor with increased trophoblast outgrowth to a greater degree than in-vivo-generated blastocysts. Murine in-vitro fertilized embryo development was not enhanced by co-culture with Vero cells, Putrescine was the most effective polyamine, enhancing the in-vitro fertilized oocyte development to blastocysts in a permissive environment.

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Documento generato il 16/01/21 alle ore 08:13:44