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Titolo:
ANTIGENIC ANALYSIS OF NEMATODE-TRANSMISSIBLE AND NON-TRANSMISSIBLE ISOLATES OF TOBACCO RATTLE TOBRAVIRUS USING MONOCLONAL-ANTIBODIES
Autore:
LEGORBURU FJ; ROBINSON DJ; TORRANCE L; DUNCAN GH;
Indirizzi:
SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND
Titolo Testata:
Journal of General Virology
, volume: 76, anno: 1995,
parte:, 6
pagine: 1497 - 1501
SICI:
0022-1317(1995)76:<1497:AAONAN>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; MOSAIC-VIRUS; EPITOPES; STRAIN; PARATRICHODORUS; TRICHODORUS; RNA-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
F.J. Legorburu et al., "ANTIGENIC ANALYSIS OF NEMATODE-TRANSMISSIBLE AND NON-TRANSMISSIBLE ISOLATES OF TOBACCO RATTLE TOBRAVIRUS USING MONOCLONAL-ANTIBODIES", Journal of General Virology, 76, 1995, pp. 1497-1501

Abstract

Murine monoclonal antibodies (MAbs) were produced against a nematode non-transmissible isolate of tobacco rattle tobravirus (TRV-PLB). Two of the MAbs (SCR 80 and 81) did not react with the serologically closely related isolate TRV-PPK20 (both isolates belong to the PRN serotype), which is readily transmissible by When further isolates of the PRN serotype were tested, all the non-transmissible ones reacted with these two MAbs, but so also did some of those that are readily transmissible. SCR 80 and 81 were able to trap TRV-PLB particles onto electron microscope grids and reacted with metatopes located near the C terminus of the particle protein. The epitope recognized by SCR 81 was discontinuous. The MAbs that reacted with both TRV-PLB and TRV-PPK20 recognized either continuous cryptotopes (SCR 78) or discontinuous neotopes (SCR 79 and 82).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:42:14