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Titolo:
CONFORMATIONAL ORDER OF SPECIFIC PHOSPHOLIPIDS IN HUMAN ERYTHROCYTES - CORRELATIONS WITH CHANGES IN CELL-SHAPE
Autore:
MOORE DJ; SILLS RH; MENDELSOHN R;
Indirizzi:
RUTGERS STATE UNIV,DEPT CHEM,NEWARK COLL ARTS & SCI,73 WARREN ST NEWARK NJ 07102 RUTGERS STATE UNIV,DEPT CHEM,NEWARK COLL ARTS & SCI NEWARK NJ 07102 CHILDRENS HOSP NEW JERSEY,DEPT PEDIAT NEWARK NJ 07107 ST BARNABAS HOSP LIVINGSTON NJ 07039 UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH NEWARK NJ 07107
Titolo Testata:
Biochemistry
fascicolo: 3, volume: 36, anno: 1997,
pagine: 660 - 664
SICI:
0006-2960(1997)36:3<660:COOSPI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORMAL-ALKYL CHAINS; H STRETCHING MODES; TRANSBILAYER MOVEMENT; BIOLOGICAL-MEMBRANES; LIPID ASYMMETRY; PROTEIN; PHOSPHATIDYLSERINE; DOMAINS; LONG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
D.J. Moore et al., "CONFORMATIONAL ORDER OF SPECIFIC PHOSPHOLIPIDS IN HUMAN ERYTHROCYTES - CORRELATIONS WITH CHANGES IN CELL-SHAPE", Biochemistry, 36(3), 1997, pp. 660-664

Abstract

Acyl chain perdeuterated dimyristoylphosphatidylcholine (DMPC-d(54)) and dimyristoylphosphatidylserine (DMPS-d(54)) were incorporated into human erythrocytes. Light microscopy demonstrated that erythrocytes incubated with an equimolar mixture of DMPC-d(54)/DMPS or DMPC/DMPS-d(54) remained mostly discocytic whereas cells incubated with either DMPC-d(54) or DMPS-d(54) alone became echinocytic or stomatocytic, respectively. Cells in which the aminophospholipid translocating protein was inhibited became echinocytic when incubated with DMPS-d(54). Fourier transform infrared (FTIR) spectroscopy was used to monitor conformational order in the acyl chains of the incorporated phospholipid, as detected through the asymmetric CD2 stretching vibrations in the intact cells. In cells incubated with equimolar mixtures of DMPC-d(54)/DMPS or DMPC/DMPS-d(54), the deuterated species exhibited no thermotropic phase transitions but revealed chain order intermediate between the gel and liquid-crystal states. In contrast, DMPS-d(54) incorporated into the outer leaflet of echinocytic erythrocytes was conformationally ordered while the same species incorporated into the inner leaflet of stomatocytic erythrocytes was highly disordered at all temperatures studied. Finally, DMPC-d(54) incorporated into the outer leaflet of echinocytic erythrocytes exhibited a phase transition, suggesting that this species persists in domains. These data indicate that the acyl chain conformational order of specific phospholipids in the intact human erythrocyte is changed with alterations in cell morphology.

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Documento generato il 28/03/20 alle ore 14:01:08